摘要
目的探究麝香保心丸(Shexiang Baoxin Pill,SBP)在小鼠缺血性脑卒中的保护作用及机制。方法通过大脑中动脉阻塞(middle cerebral artery occlusion,MCAO)构建脑缺血性卒中模型,将96只C57BL/6小鼠随机分成4组:假手术(Sham)组、模型(ischemia/reperfusion,I/R)组、低剂量麝香保心丸(LSBP,22.5 mg/kg)组和高剂量麝香保心丸组(HSBP,45 mg/kg),每组24只。灌胃4周后制备小鼠MCAO模型。记录各组小鼠的大脑梗死体积及脑组织含水量,利用免疫荧光技术分析激活的小胶质细胞数量,采用q RT-PCR检测炎症因子的表达,通过Western blot法分析NF-κB信号通路相关蛋白质的表达情况。结果与I/R组相比,LSBP组和HSBP组大脑梗死体积减少,脑组织含水量降低,激活的小胶质细胞数量减少,同时促进小胶质细胞由M1型向M2型极化,并抑制NF-κB信号通路的激活。结论SBP可以减轻由缺血性脑卒中引起的小鼠脑组织损伤,其机制可能与改变小胶质细胞M1/M2的极化状态和抑制NF-κB信号通路的激活有关。
Objective To explore the protective effect and mechanism of Shexiang Baoxin pill(SBP)on ischemic stroke in mice.Methods Ischemic stroke was established by middle cerebral artery occlusion(MCAO)in C57 BL/6 mice.A total of 96 mice were randomly divided into 4 groups(n=24 for each group):sham operation group(Sham group),MCAO with normal saline group(I/R group),MCAO with low-dose(22.5 mg/kg)SBP group(LSBP group)and MCAO with high-dose(45 mg/kg)SBP group(HSBP group).MCAO mouse model was established after 4 weeks of intragastric administration.The infarct size and brain water content were recorded in all groups,the number of activated microglia was analyzed by immunofluorescence(IF),the expression of inflammatory factors was detected by qRT-PCR,and the protein expression within NF-κB signal pathway was analyzed by Western blot.Results Compared with I/R group in LSBP group and HSBP group,the infarct size and brain water content reduced,the number of activated microglia reduced,the polarization of microglia from M1 phenotype to M2 phenotype was promoted,and the activation of NF-κB signaling pathway was attenuated.Conclusion SBP protected against brain injury caused by ischemic stroke in mice.The mechanism may relate to transforming the polarization of microglia and attenuating the activation of NF-κB signaling pathway.
作者
邵帅
陈彦霖
地里热巴提·地力木拉提
贾锋
SHAO Shuai;CHEN Yan-lin;DILIMULATI Dilirebati;JIA Feng(Department of Neurosurgery,Renji Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200127,China)
出处
《复旦学报（医学版）》
CAS
CSCD
北大核心
2021年第3期292-299,共8页
Fudan University Journal of Medical Sciences
基金
国家自然科学基金(81471855)。
