摘要
目的探讨海马γ振荡异常在脓毒症相关性脑病(sepsis-associated encephalopathy,SAE)中的作用。方法成年雄性Sprague-Dawley大鼠(2~3个月龄)70只,采用盲肠结扎穿孔术(cecal ligation and puncture,CLP)建立SAE动物模型,根据随机数字表法,随机分为三组:假手术组(sham组),CLP组及CLP+多巴胺4(dopamine 4,D4)受体激动剂RO-10-5824组。于术后第10~14天分别行旷场、新物体识别及条件恐惧实验;行为学结束后取海马组织检测微清蛋白(parvalbumin,PV)与D4受体表达情况;行海马CA1场电位检测,并分析其与大鼠新事物探索时间的关系。计量资料两两比较采用独立t检验,多组间的差异采用单因素方差分析,多重比较采用Tukey法。相关性分析采用Pearson法。以P<0.05为差异有统计学意义。结果与对照组相比,CLP组海马PV(77.54±4.61)%、D4受体(56.36±3.88)%及γ振荡能量(41.1±8.62)%,新事物接触时间(36±3)s、新事物识别率(49±4)%与场景僵直时间(56±7)s均显著降低(P<0.05),而RO-10-5824可上调γ振荡能量(92.3±6.7)%,逆转降低的新事物接触时间(44±3)s和新事物识别率(63±4)%。相关分析显示海马CA1区γ振荡能量与新事物接触时间成正相关(r=0.6092,P=0.0159)。然而,各组动物在总探索路程和中央格停留时间差异无统计学意义(P>0.05)。结论海马γ振荡异常在SAE的认知功能障碍中发挥关键作用。
Objective To explore the role of hippocampalγoscillation abnormality in sepsisassociated encephalopathy(SAE).Methods Seventy male Sprague-Dawley rats(2-3 months)were randomly(random number)divided into three groups according to the random digital table method:sham,CLP,and CLP+dopamine 4(D4)receptor agonists RO-10-5824 group.The SAE animal model was established by cecal ligation and puncture(CLP).On day 10-14 after surgery,the open field,novel object recognition,and fear conditioning tests were performed.After that,the hippocampus was collected to measure expressions of parvalbumin(PV)and D4 receptor.In another set of experiment,CA1 local field potential(LFP)were recorded,and the relationship between LFP and time with novel object was analyzed.Independent sample t-test was used for pairwise comparisons,and multiple comparisons were performed by one-way ANOVA,followed by the Tukey multiple comparisons test.Correlation was analyzed using Pearson correlation.Statistical significance was assumed when P<0.05.Results Compared with the sham group,hippocampal PV(77.54±4.61)%,D4 expression(56.36±3.88)%andγoscillation power(41.1±8.62)%,object exposure time(36±3)s,new object recognition rate(49±4)%,and scene stiffness time(56±7)s were decreased significantly(P<0.05).However,RO-10-5824 treatment could increase hippocamlγoscillation power(92.3±6.7)%,and reverse the decreased new object exposure time(44±3)s and new object recognition rate(63±4)%.Correlation analysis showed that hippocampalγoscillation power was positively associated with new object exposure time(r=0.6092,P=0.0159).There was no difference in total distance traveled or time spent in the center among groups(P>0.05).Conclusion Hippocampalγoscillation abnormality might play a key role in cognitive impairment associated with SAE.
作者
范云霞
李国民
戴雷
谷书馨
张晶云
尹琪
高戎
Fan Yunxia;Li Guomin;Dai Lei;Gu Shuxin;Zhang Jingyun;Yin Qi;Gao Rong(Department of Anesthesiology,Jintan Hospital,Jiangsu University,Changzhou 213200,China;Department of Intensive Care Unit,Jintan Hospital,Jiangsu University,Changzhou 213200,China;Department of Emergency,Nanjing Integrated Traditional Chinese and Western Medicine Hospital,Nanjing University of TCM,Nanjing 210014,China)
出处
《中华急诊医学杂志》
CAS
CSCD
北大核心
2021年第5期557-561,共5页
Chinese Journal of Emergency Medicine
基金
国家自然科学基金(81701884)
江苏省卫生厅重大科研项目(YG201409)
常州市科技计划(应用基础研究)(CJ20200001)。
关键词
γ振荡
脓毒症
微清蛋白
场电位
认知功能障碍
大鼠
γoscillation
Sepsis
Parvalbumin
Local field potential
Cognitive impairment
Rats
