摘要
探讨高原低氧环境对大鼠肠道菌群结构和多样性的影响。动物实验严格遵循青海大学医学院医学伦理委员会的规定。SD大鼠随机分为平原对照组、中度海拔缺氧组和高度海拔缺氧组,缺氧组大鼠分别于低氧暴露第3、7、15、30天测定粪便pH值,HE染色法观察小肠组织病理形态学变化,16S rDNA高通量测序技术对肠道菌群进行测序。与平原对照组相比,中度海拔缺氧组和高度海拔缺氧组大鼠粪便pH值均显著降低,高原低氧环境对小肠组织具有一定的影响,中度海拔缺氧组在低氧暴露第3天固有层和黏膜下层毛细血管轻度扩张充血,高度海拔缺氧组在低氧暴露第7天黏膜下层毛细血管扩张充血、黏膜固有层轻度水肿、淋巴管扩张。高原低氧环境下,大鼠肠道菌群的结构和多样性随低氧暴露时间延长发生显著改变。大鼠肠道菌群共检测出35个门、87个纲、205个目、337个科、638个属和256个种,其中厚壁菌门、梭菌纲、梭菌目、瘤胃菌科、阿克曼菌属和鼠乳杆菌相对丰度较高且具有统计学意义。与平原对照组相比,缺氧组大鼠肠道菌群在低氧暴露第15天差异最明显,中度海拔缺氧组中相对丰度较高差异菌有9个,以理研菌科_RC9_gut_group为主,高度海拔缺氧组中相对丰度较高的差异菌有19个,以瘤胃菌科为主。研究结果对明确肠道菌群与高原低氧的关系具有指导意义,为进一步研究高原低氧条件下疾病的形成和发展及药物代谢提供理论依据。
The structure and diversity of the intestinal flora in rats exposed to high altitude hypoxia was investigated.Animal experiments strictly follow the regulations of Medical Laboratory Animal Ethics Committee of Qinghai University,School of Medicine.SD rats were randomly divided into a control group,a moderate altitude hypoxia group,and a high altitude hypoxia group.The pH value of the feces was measured and histopathological changes in the small intestine were determined by HE staining,and the intestinal flora were characterized by 16S rDNA high throughput sequencing technology on the 3rd,7th,15th,and 30th day of hypoxia exposure.Compared with the control group,the fecal pH value of rats in the moderate altitude hypoxia group and the high altitude hypoxia group was decreased significantly.The lamina propria and submucosa capillaries were slightly dilated and congested on the 3rd day in the moderate altitude hypoxia group.In the high altitude hypoxia group the submembrane capillaries were dilated and congested,the lamina propria of the mucosa showed mild edema,and the lymphatic vessels were dilated on the 7th day.The composition and diversity of intestinal flora in these rats changed significantly with prolonged exposure to the high altitude hypoxic environment.A total of 35 phyla,87 classes,205 orders,337 families,638 genera,and 256 species were annotated in the three groups of rats,including Firmicutes,Clostridia,Clostridiales,Ruminococcaceae,Akkermansia,and Lactobacillus_murinus.Compared with the control group,the intestinal flora of the hypoxic groups showed the most significant changes by the 15th day.There were 9 microbiota of gut microorganisms with relative abundance in the moderate altitude hypoxia group,of which Rikenellaceae_RC9_gut_group bacteria was the most common,there were 19 different microbiota of gut microorganisms with higher relative abundance in the high altitude hypoxia group,of which Ruminococcaceae bacteria was the most common.The results of this study indicate significant changes in the in
作者
刘贵琴
白雪
段雅彬
朱俊博
杨建鑫
王倩
周杨
顾文琦
李向阳
LIU Gui-qin;BAI Xue;DUAN Ya-bin;ZHU Jun-bo;YANG Jian-xin;WANG Qian;ZHOU Yang;GU Wen-qi;LI Xiang-yang(College of Eco-Environmental Engineering,Qinghai University,Xining 810016,China;Research Center for High Altitude Medicine,Qinghai University,Xining 810001,China;Medical College of Qinghai University,Xining 810001,China;State Key Laboratory of Plateau Ecology and Agriculture,Qinghai University,Xining 810016,China)
出处
《药学学报》
CAS
CSCD
北大核心
2021年第4期1100-1108,共9页
Acta Pharmaceutica Sinica
基金
国家自然科学基金资助项目(81760673,81460568)
青海省创新平台建设专项(2021-ZJ-T03)。
关键词
高原
低氧
肠道菌群
16S
rDNA
差异菌
多样性
plateau
hypoxia
intestinal flora
16S rDNA
differential microbiota of gut microorganisms
diversity
