摘要
目的 研究测定肠道黏膜屏障功能的几种方法及评价不同方法之间的相关性.方法 16只无特定病原体动物（SPF）级雄性SD大鼠,随机分为对照组（Control）和缺血/再灌注（L/R）组,每组8只.适应性饲养5d后,I/R组大鼠进行肠道缺血60 min,Control组只开腹60 min,然后继续饲养1d后取材.检测两组血浆二胺氧化酶（DAO）、D-乳酸（D-LAC）、内毒素、谷氨酰胺（Gln）的浓度,观察小肠黏膜形态学.术前1d和术后1d分别测定肠道通透性乳果糖/甘露醇（L/M）.结果 I/R组血浆DAO、D-LAC和内毒素水平显著高于Control组[DAO：（0.498±0.032） U/ml比（0.247±0.051） U/ml,t=-11.790,P=0.000; D-LAC：（5.47±1.55） mg/L比（3.83 ±0.63） mg/L,t=-2.757,P=0.022;内毒素：（0.039 5±0.002 8） EU/ml比（0.025 6±0.004 5） EU/ml,t=-7.377,P=0.000];血浆Gln浓度显著低于Control组,为（646.12±34.75）μmol/L比（839.13±163.76） μmol/L（t=3.261,P=0.012）;I/R组术后1 dL/M值明显高于术前1d,为3.63±2.09比1.22±0.66（t=-3.118,P=0.013）.术后I/R组的空肠黏膜厚度、空肠绒毛高度、回肠黏膜厚度和回肠绒毛高度明显低于Control组,分别为（329.80±64.68） μm比（512.82±38.41）μm （t=6.881,P=0.000）,（253.06±69.33） μm比（386.79 ±56.39） μm（t=4.232,P=0.001）,（205.89±18.71） μm比（335.29±27.71） μm （t=10.945,P=0.000）,（135.61±22.30） μm比（253.18 ±31.02） μm（t=8.705,P=0.000）.I/R组肠道缺血/再灌注后血浆DAO、D-LAC、内毒素及L/M均增高,DAO与D-LAC（r=0.971,P＜0.01）、内毒素（r=0.915,P＜0.01）及L/M（r=0.957,P＜0.01）相互之间呈正相关;缺血/再灌注后血浆Gln浓度降低,与DAO（r=-0.964,P＜0.01）、D-LAC（r=-0.966,P＜0.01）、内毒素（r=-0.927,P＜0.01）和L/M（r=-0.993,P＜0.01）分别呈负相关.结论 肠道缺血/再灌注后,各项指标均有明显改变并具有良好的相关性,DAO与D-LAC、内毒素及L/M间呈显著正相关,与肠道屏障功能呈�
Objective To study several measuring methods of the intestinal mucosa barrier and evaluate the correlation between different methods for determining the intestinal damage.Methods Sixteen specific pathogen free （SPF） male Sprague-Dawley rats were randomly divided into two groups：the Control group （n =8） and the ischemia/reperfusion （I/R） group （n =8）.After adaptive feeding for 5 days,I/R group was put into ischemia model for 60 min and the Control group was merely opened on its abdominal wall but without ischemia for 60 min.After having been fed for another day,all rats were killed and specimens were collected.The plasma diamine oxidase （DAO）,D-lactate （D-LAC）,endotoxin,and glutamine （Gln） levels were detected,and the intestinal mucosal morphology was observed.The intestinal permeability （L/M） was detected 1 day before and after the surgery.Results The plasma DAO,D-LAC,and endotoxin levels were significantly higher in I/R group compared with the Control group （DAO：（0.498 ±0.032） vs （0.247 ±0.051） U/ml,t=-11.790,P=0.000; D-LAC：（5.47±1.55） vs （3.83±0.63） mg/L,t=-2.757,P=0.022; endotoxin：（0.0395±0.002 8） vs （0.025 6 ±0.004 5） EU/ml,t =-7.377,P =0.000）.The plasma Gln concentration was significantly lower than that in the Control group [（646.12 ± 34.75） vs （839.13 ± 163.76） μmol/L,t =3.261,P =0.012）.The L/M value on the 1 st postoperative day was significantly higher than that in the I/R group [（3.63 ±2.09） vs （1.22 ±0.66）,t =-3.118,P =0.013）].The jejunum mucosal thickness,jejunum villus height,ileal mucosal thickness,and ileal villus height were significantly lower in I/R group compared with the Control group after operation [（329.80 ±64.68） vs （512.82 ±38.41） μm,t=6.881,P=0.000; （253.06±69.33） vs （386.79±56.39） μm,t=4.232,P=0.001; （205.89± 18.71） vs （335.29±27.71） μm,t=10.945,P=0.000; （135.61 ±22.30） vs （253.18±31.02） μm,t =8.705,P =0.000].After intestinal ischemia/reperfusion
出处
《中华临床营养杂志》
CAS
CSCD
2014年第4期213-218,共6页
Chinese Journal of Clinical Nutrition
基金
基金项目：国家自然科学基金（30471707）
