摘要
从临床病料中分离纯化1株猪伪狂犬病毒——HuB2020,并对其毒力、培养特性及遗传变异开展了一系列研究。结果表明,该毒株gE基因核苷酸序列与国内PRV毒株的核苷酸序列同源性在98.9%~99.9%。纯化后的病毒经测定其在PK15细胞上的TCID_(50)为10^(7.5)/mL;对6周龄昆明小鼠的LD_(50)为1×10^(5.7)。组织嗜性分布显示,10^(6.0)TCID_(50)/0.1 mL、10^(5.0)TCID_(50)/0.1 mL攻毒组对小鼠的神经嗜性均高达100%,且病毒在脑和心脏中的检出率均高于其他组织。
A strain of porcine pseudorabies virus:HuB2020 was isolated and purified from clinical materials,and a series of studies were carried out on its virulence,culture characteristics and genetic variation.The results showed thatthe nucleotide sequence of gE gene in this strain was 98.9%~99.9%with that of PRV(Pseudorabies virus,PRV)strain in China.TCID_(50) of purified virus on PK15 cells was determined to be 10^(7.5)/mL.The LD_(50) of 6-week-old Kunming mice was 1×10^(5.7).Tissue sexual distribution showed that,10^(6.0)TCID_(50)/0.1 mL,10^(5.0) TCID_(50)/0.1 mL challenge groups showed 100%neurotropism to mice,and the detection rate of the virus in brain tissue and heart tissue was higher than other tissues.
作者
翟瑶
周丹娜
梁婉
杨克礼
袁芳艳
郭锐
李鹏
田永祥
ZHAI Yao;ZHOU Dan-na;LIANG Wan;YANG Ke-li;YUAN Fang-yan;GUO Rui;LI Peng;TIAN Yong-xiang(College of Animal Science,Yangtze University,Jingzhou 434025,Hubei,China;Key Laboratory of Preparation for Prevention and Control of Livestock and Poultry Bacterial Diseases,Ministry of Agriculture and Rural Affairs/Institute of Animal Science and Veterinary Medicine,Hubei Academy of Agricultural Sciences,Wuhan 430064,China)
出处
《湖北农业科学》
2021年第10期105-109,共5页
Hubei Agricultural Sciences
基金
国家重点研发计划项目(2018YFD0500800)
湖北省科技重大专项(2020ABA016)。
关键词
猪伪狂犬病病毒
分离
鉴定
遗传进化分析
porcine pseudorabies virus
separation
identification
genetic evolutionary analysis
