摘要
为了解猪伪狂犬病病毒(porcine pesudorabies virus,PRV)gB和gE基因变异及遗传演化情况,本研究针对PRV FS-2015野毒株,应用"蚀斑法"对组织病料中病毒进行三轮纯化,应用全长扩增引物对FS-2015株gB和gE基因进行全基因扩增,并对PCR产物进行测序和序列分析。结果显示,PRV FS-2015株的gB、gE基因与国内外PRV参考毒株的核苷酸同源性分别为97.0%～100.0%和97.5%～99.7%,氨基酸同源性分别为96.4%～100.0%和95.3%～99.7%。氨基酸变异位点分析表明,FS-2015株的gB和gE基因均有位点突变和缺失。遗传进化分析表明,FS-2015株与国内近几年分离的PRV变异株GY、ZJ01、HB1201、HN1201、JS2012、BJ-YT和BP属于同一分支,同源性较高,亲缘关系较近;与PRV经典株Kaplan、Becker、NIA3、Kolchis、Bartha和Yangsan株属于不同分支,同源性较低,亲缘关系较远。从PRV FS-2015毒株与国内外经典毒株和当前国内流行的变异毒株的分析结果可知,PRV FS-2015毒株发生了一定的变异,属于当前国内流行变异毒株。本研究结果为广东省伪狂犬病分子流行病学调查、伪狂犬病的防控和疫苗株挑选工作提供参考数据。
To study the genetic mutation and evolution of gBand gEgenes of porcine pesudorabies virus(PRV),the FS-2015 wild strain PRV was purified in the tissue disease by plaque assay method(three times),the whole gene fragments of gBand gE genes were amplified,and PCR products were sequenced and analyzed in this study.The results showed that the homology of nucleotide sequence of gBand gEgenes of PRV FS-2015 strain with other PRV strains from GeneBank were 97.0%to 100.0% and 97.5%to 99.7%,the homology of amino acid sequence were96.4%to 100.0%and 95.3%to 99.7%,respectively.The amino acid variation site analysis results showed that gBand gEgenes of FS-2015 strain had site mutations and deletions.Phylogenetic tree analysis results showed that the PRV FS-2015 stain with GY,ZJ01,HB1201,HN1201,JS2012,BJ-YT and BP stains had closer relationship,while that with Kaplan,Becker,NIA3,Kolchis,Bartha and Yangsan strains had a distant relationship.The results of PRV FS-2015 strains and classical strains at home and abroad,and the currently domestic variant strains suggested that PRV FS-2015 strain belonged to the variant popular in recent years,which could provide data for the molecular epidemiological investigation,the prevention and control of pseudorabies and the selection of vaccine strains in Guangdong province.
作者
庞旋飞
练斯南
伍建敏
万曾培
王征帆
李中圣
白挨泉
PANG Xuanfeiis;LIAN Sinan;WU Jianmin;WAN Zengpei;WANG Zhengfan;LI Zhongsheng;BAI Aiquan(Foshan University,Foshan 528231, China;Guangdong Haid Institute of Animal Husbandry and Veterinary , Guangzhou 511400, China)
出处
《中国畜牧兽医》
CAS
北大核心
2018年第12期3524-3534,共11页
China Animal Husbandry & Veterinary Medicine
基金
广东省教育厅预防兽医学重点实验室项目(2014KTSPT037)
广东省省级科技计划项目(2015B020203006)
