摘要
南方地区番茄在春延后栽培和秋提前栽培中易受异常高温的影响,采用耐热砧木进行嫁接栽培可以提高番茄的耐热性。为筛选耐热性强的番茄砧木种质资源,本试验对20份番茄砧木种质的种子和幼苗进行热胁迫处理,根据热害表型和幼苗生长生理指标评价砧木种质的耐热性。结果表明,热胁迫抑制番茄砧木种子萌发,随着温度升高,种子发芽率明显降低。34℃时,除N3外的砧木种质种子发芽率降至50%以下;37℃时种子发芽率均降至24%以下,40℃时种子发芽率均为0。亚高温(34℃/28℃)和高温(37℃/28℃)胁迫下,G3、N3、Q2均表现强耐高温(HR),A1、Q3、Q4、Q5、Y4表现中耐高温(MR)或耐高温(R)。幼苗热害指数与株高、叶片丙二醛(MDA)含量呈极显著正相关,与叶片SPAD值、茎粗、全株鲜质量、全株干质量、叶片游离脯氨酸含量呈显著或极显著负相关。利用以上7项生长生理指标,采用隶属函数值法对供试种质的耐热性进行综合评价,种质G3、N3、Q2的耐热性居前3位,与亚高温胁迫的幼苗热害指数排序一致。综上,种质G3、N3、Q2强耐高温,可作为选育耐热番茄砧木的骨干材料。
In southern China,tomato is susceptible to abnormal high temperature in late spring cultivation and early autumn cultivation.Tomato heat resistance can be improved by grafting cultivation with heat-resistant rootstock.In order to screen the germplasm resources of tomato rootstock with strong heat resistance,this study conducted heat stress treatment on 20 tomato rootstock seeds and seedlings,and evaluated the heat resistance of these rootstock germplasm according to the heat damage phenotype and physiological indexes of seedling growth.The results showed that heat damage had restrained the seed germination of tomato rootstock.Along with the temperature rising,germination rate was significantly reduced.The germination rate of rootstock seeds mostly dropped to below 50%at 34℃,below 23%at 37℃,and to 0 at 40℃.‘G3’,‘N3’and‘Q2’showed strong high temperature resistance(HR)under subhigh temperature(34℃/28℃)and high temperature(37℃/28℃)stress,while‘A1’,‘Q3’,‘Q4’,‘Q5’and‘Y4’showed moderate high temperature resistance(MR)or high temperature resistance(R).The seedling heat damage index was extremely positively correlated with plant height and MDA content in leaves,and significantly or extreme significantly negatively correlated with SPAD value,stem diameter,fresh weight and dry weight of the whole plant,and free proline contents of leaves.According to the above 7 growth physiological indexes,the heat resistance of these tested germplasm was comprehensively evaluated by subordinate function value method.And the heat resistance of‘G3’,‘N3’and‘Q2’was ranked from 1-3,Which was consistent with the heat damage indexes of seedlings under sub-high temperature stress.In summary,‘G3’,‘N3’and‘Q2’were strong heat tolerant germplasm resources,and could be used as backbone material for selective breeding of heat-resistant tomato rootstocks.
作者
田茂燕
向婷颖
钟川
王鹏
阳燕娟
于文进
TIAN Maoyan;XIANG Tingying;ZHONG Chuan;WANG Peng;YANG Yanjuan;YU Wenjing(College of Agronomy,Guangxi University,Nanning 530004,Guangxi,China)
出处
《中国蔬菜》
北大核心
2021年第5期39-47,共9页
China Vegetables
基金
国家自然科学基金项目(31660568)
广西科技重大专项(桂科AA17204039-2,AA17204026-1)。
关键词
番茄
砧木
耐热性
生理响应
tomato
rootstock
heat resistance
physiological response
