摘要
目的:探讨前列腺癌病理分级与α-甲酰基辅酶A-消旋酶、人高分子量细胞角蛋白、p63蛋白及S-100蛋白的关系。方法:收集2017年1月~2020年10月前列腺穿刺活检标本,前列腺癌标本92例,纳入PCa组;良性前列腺增生标本80例,纳入BPH组。所有标本均行免疫组化检测α-甲酰基辅酶A-消旋酶和人高分子量细胞角蛋白、p63、S-100蛋白的表达,光镜下观察前列腺癌患者病理组织学形态,进行Gleason分级评分。比较α-甲酰基辅酶A-消旋酶、人高分子量细胞角蛋白、p63、S-100蛋白在PCa组、BPH组中的表达阳性率,以及在不同Gleason分级中的表达强度,采用Spearman相关性分析α-甲酰基辅酶A-消旋酶水平、人高分子量细胞角蛋白、p63、S-100蛋白表达强度与Gleason分级的相关性。结果:α-甲酰基辅酶A-消旋酶在PCa组中表达阳性率高于BPH组,人高分子量细胞角蛋白、p63、S-100蛋白在PCa组中表达阳性率低于BPH组(P均<0.05);PCa组中Gleason评分≤7分者α-甲酰基辅酶A-消旋酶表达强度低于Gleason评分>7分者(P<0.05),人高分子量细胞角蛋白、p63、S-100蛋白在PCa组不同Gleason分级中的表达强度差异无统计学意义(P>0.05)。Spearman相关性分析结果显示,Gleason分级与α-甲酰基辅酶A-消旋酶表达强度呈正相关性(r=0.632,P<0.05),与人高分子量细胞角蛋白(r=0.212,P>0.05)、p63(r=0.197,P>0.05)、S-100蛋白(r=0.244,P>0.05)表达强度无显著相关性。结论:α-甲酰基辅酶A-消旋酶、人高分子量细胞角蛋白、p63、S-100蛋白在前列腺癌的鉴别诊断中有重要应用价值,α-甲酰基辅酶A-消旋酶可作为评价前列腺癌病情与预后的重要参考指标。
Objective:To explore the relationship between pathological grades of prostate cancer and α-formyl coenzyme A-racemase,Human high molecular weight cytokeratin,p63 protein and S-100 protein.Methods:From January 2017 to October 2020,92 prostate cancer specimens were included in PCa group;80 benign prostatic hyperplasia specimens were included in BPH group.The expressions of α-formyl coenzyme A-racemase,Human high molecular weight cytokeratin,p63 protein,S-100 protein were detected by immunohistochemistry.The histopathological morphology of prostate cancer patients was observed under light microscope,and Gleason grading was performed.The levels of α-formyl coenzyme A-racemase,the positive rates of Human high molecular weight cytokeratin,p63 protein and S-100 protein expression in PCa group and BPH group,and the expression intensity of these indicators in different Gleason grades were compared.The correlation between α-formyl coenzyme A-racemase,Human high molecular weight cytokeratin,p63 protein,S-100 protein expression intensity and Gleason grades were analyzed by Spearman correlation analysis.Results:The positive rate of α-formyl coenzyme A-racemase expression in PCa group was higher than that in BPH group,while the positive rates of Human high molecular weight cytokeratin,p63 protein and S-100 protein expression in PCa group were lower than those in BPH group(all P<0.05).The expression intensity of α-formyl coenzyme A-racemase of patients with Gleason score≤7 points in PCa group was lower than that of patients with Gleason score>7 points(all P<0.05);but there was no significant difference in the expression intensity of Human high molecular weight cytokeratin,p63 and S-100 protein in different Gleason grades of PCa group(P>0.05).Spearman correlation analysis showed that Gleason grade was positively correlated with α-formyl coenzyme A-racemase expression intensity(r=0.632,P<0.05),but not with Human high molecular weight cytokeratin(r=0.212,P>0.05),p63(r=0.197,P>0.05)and S-100 protein(r=0.244,P>0.05).Conclus
作者
段凯军
李青
DUAN Kai-jun;LI Qing(The Department of Pathology,the Fourth People's Hospital of Shangqiu,Henan,Shangqiu 476100)
出处
《实用中西医结合临床》
2021年第4期12-14,49,共4页
Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine
