摘要
目的:探讨子痫前期产妇外周血和胎盘中胎盘生长因子(PLGF)和可溶性血管内皮生长因子受体-1(sVEGFR-1)的相关性及临床意义。方法:选择2017年1月—2019年12月在本院妇产科住院的轻度子痫前期(轻度组)、重度子痫前期孕产妇(重度组)各56例,同期本院就诊正常孕产妇56例(正常组),酶联免疫吸附试验法检测产妇分娩时外周血清PLGF和sVEGFR-1蛋白水平,PR-PCR测定胎盘PLGF和sVEGFR-1 mRNA水平,分析孕产妇病情及妊娠结局。结果:血清和胎盘组织中PLGF蛋白水平均重度组<轻度组<正常组,血清和胎盘组织中sVEGFR-1蛋白水平重度组>轻度组>正常组(均P<0.05)。子痫前期患者血清PLGF和胎盘PLGF蛋白水平与24h蛋白尿、收缩压和舒张压均负相关,血清和胎盘sVEGFR-1蛋白水平与24h蛋白尿、收缩压和舒张压均正相关(均P<0.05)。正常组、轻度组和重度组分别有1例、6例和15例围生儿出现临床结局不良(P<0.05),妊娠结局不良产妇外周血清和胎盘PLGF mRNA水平均较妊娠结局正常孕妇低,血清和胎盘sVEGFR-1蛋白水平较妊娠结局正常孕妇高(均P<0.05)。结论:子痫前期孕产妇外周血和胎盘组织PLGF和sVEGFR-1水平可能与PE病情程度及围产儿结局有相关性。
Objective:To investigate the correlation and clinical significance of placental growth factor(PLGF)and soluble vascular endothelial growth factor receptor-1(sVEGFR-1)in peripheral blood and placenta of pregnant women with pre-eclampsia.Methods:From January 2017 to December 2018,56 pregnant women with severe pre-eclampsia were included in group A,56 pregnant women with mild pre-eclampsia were selected in group B,and 56 normal pregnant women were selected in group C.The protein levels of PLGF and sVEGFR-1 in peripheral blood were detected by enzyme-linked immunosorbent assay(ELISA).The protein levels of PLGF and sVEGFR-1 mRNA in placenta was determined by PR-PCR.The state of disease and pregnancy outcomes of these women was analyzed.Results:The PlGF protein level in serum and placental tissues of the women in group A was the lowest,and that of the women in group C was the highest.The SVEGFR-1 protein level in serum and placental tissues of the women in group A was the highest,and that of the women in group C was the lowest(all P<0.05).The protein levels of PLGF in serum and placental of the women with pre-eclampsia were negatively correlated with their 24h proteinuria value,and systolic and diastolic blood pressure.The protein levels of SVEGFR-1 in serum and placental of the women with pre-eclampsia were positively correlated with their 24h proteinuria,and systolic and diastolic blood pressure(all P<0.05).There were 1 perinatal infant with adverse clinical outcomes in group C,6 perinatal infants with adverse clinical outcomes in group B,and 15 perinatal infants with adverse clinical outcomes in group A(P<0.05).The levels of PLGF mRNA in serum and placental of the women with adverse pregnancy outcomes were significant lower than those of women with normal pregnancy outcomes,but the levels of SVEGFR-1 protein in serum and placental of the women with adverse pregnancy outcomes were significant higher than those of women with normal pregnancy outcomes(all P<0.05).Conclusion:The levels of PLGF and sVEGFR-1 in peripheral
作者
闵治红
杜巧玲
MIN Zhihong;DU Qiaoling(The First Maternity and Infant Hospital Affiliated to Tongji University School of Medicine, Shanghai, 201204)
出处
《中国计划生育学杂志》
2021年第2期350-354,共5页
Chinese Journal of Family Planning
关键词
子痫前期
胎盘生长因子
可溶性血管内皮生长因子受体-1
围产儿结局
Pre-eclampsia
Placental Growth Factor
Soluble Vascular Endothelial Growth Factor Receptor-1
Outcomes of perinatal infant
