摘要
目的探讨血清miR-21、miR-125b在子痫前期患者中的相对表达水平变化及其临床意义。方法以2018年7月至2019年10月在上海中医药大学附属曙光医院诊治的子痫前期孕妇60例为研究对象,其中轻度子痫前期患者28例为轻度组,重度子痫前期患者32例为重度组。另选取同期健康妊娠孕妇58例为对照组。检测孕妇血清miR-21、miR-125b的相对表达水平及其他临床指标,采用Pearson相关进行相关性分析,并采用受试者工作特征曲线(ROC曲线)分析血清miR-21、miR-125b对子痫前期的诊断价值。结果轻度组、重度组子痫前期患者收缩压、舒张压均较对照组明显升高,新生儿体质量明显下降(P<0.05);重度组子痫前期患者收缩压、舒张压明显高于轻度组(P<0.05),新生儿体质量明显低于轻度组(P<0.05)。轻度组子痫前期患者血清miR-21、miR-125b的相对表达水平明显高于对照组(P<0.05);而重度组子痫前期患者血清miR-21、miR-125b的相对表达水平明显高于轻度组(P<0.05)。孕妇血清miR-21、miR-125b与收缩压、舒张压、24 h蛋白尿、血清肌酐、尿素氮呈正相关(rmiR-21=0.208、0.503、0.649、0.539、0.457,均P<0.05;rmiR-125b=0.273、0.444、0.522,0.380、0.364,均P<0.05),与新生儿体质量呈负相关(rmiR-21=-0.249,P<0.05;rmiR-125b=-0.207,P<0.05)。ROC曲线结果显示,血清miR-21、miR-125b对子痫前期联合检测的诊断效能高于单独检测,差异有统计学意义(P<0.05)。结论血清miR-21与miR-125b在子痫前期患者中相对表达水平升高,对子痫前期具有一定的诊断价值。
Objective To investigate the relative expression levels and clinical significance of serum miR-21 and miR-125b in patients with preeclampsia.Methods From July 2018 to October 2019,60 pregnant women with preeclampsia who were treated in Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine were selected as the research objects,including 28 cases of mild preeclampsia(mild group)and 32 cases of severe preeclampsia(severe group).Another 58 healthy pregnant women in the same period were selected as the control group.The relative expression levels of serum miR-21,miR-125b and other clinical indicators were detected.Pearson correlation was used to analyze the correlation.The diagnostic value of serum miR-21 and miR-125b in preeclampsia was analyzed by receiver operating characteristic curve(ROC).Results Compared with the control group,the systolic blood pressure and diastolic blood pressure of the mild group and the severe group were significantly increased,and the weight of the newborn was significantly decreased(P<0.05);the systolic blood pressure and diastolic blood pressure of the severe group were significantly higher than those of the mild group(P<0.05),and the weight of the newborn was significantly lower(P<0.05).The relative expression levels of miR-21 and miR-125b in mild group were significantly higher than those in control group(P<0.05),while those in severe group were significantly higher than those in mild group(P<0.05).The serum miR-21,miR-125b levels were positively correlated with systolic blood pressure,diastolic pressure,24-hour proteinuria,creatinine and blood urea levels(rmiR-21=0.208,0.503,0.649,0.539,0.457,all P<0.05;rmiR-125b=0.273,0.444,0.522,0.380,0.364,all P<0.05),and were negatively correlated with the body mass of the newborn(rmiR-21=-0.249,P<0.05;rmiR-125b=-0.207,P<0.05).ROC curve results showed that the combined diagnosis efficacy of serum miR-21 and miR-125b in preeclampsia was higher than that of single diagnosis,and the difference was statistically significa
作者
袁媛
韩大鹏
胡丽
UAN Yuan;HAN Dapeng;HU Li(School of Health and Social Care,Shanghai Urban Construction Vocational College,Shanghai 201415,China;Department of Joint Surgery,Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai 200000,China;Department of Obstetrics and Gynecology,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200021,China)
出处
《国际检验医学杂志》
CAS
2021年第9期1041-1045,共5页
International Journal of Laboratory Medicine
基金
国家自然科学基金项目(81670232)。
