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大型海藻类菌孢素氨基酸提取工艺及定性检测研究 被引量:5

Extraction Process and a Qualitative Detection Method of Mycosporine-Like Amino Acids from Six Species of Marine Macroalgae
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摘要 类菌孢素氨基酸(Mycosporine-like amino acids,MAAs)是一类多功能活性化合物,在大型海藻中广泛存在。以海带、裙带菜、羊栖菜、江蓠、石花菜和海萝为原料,通过一系列单因素和正交实验,建立了此6种大型海藻MAAs的适宜提取工艺,并建立了定性检测方法。结果表明,海带、裙带菜、羊栖菜、江蓠、石花菜和海萝MAAs的适宜提取温度、固液比和甲醇体积分数依次为35、30、30、30、30、25 ℃,1:60、1:60、1:60、1:60、1:60、1:50 g/mL,30%、10%、30%、10%、30%、10%。利用上述工艺条件,制备得到6种大型海藻MAAs提取物,得率分别为(39.0±3.80)%、(20.6±2.20)%、(32.8±2.60)%、(5.41±1.20)%、(9.87±0.80)%、(16.2±1.40)%。在此基础上,250~390 nm波长扫描显示6种大型海藻MAAs提取物均在330 nm附近有吸收,表明它们中存在Porphyra-334。进一步,初次确定了海带、裙带菜、羊栖菜、江蓠、石花菜和海萝中Porphyra-334含量,依次为(1.60±0.69)μg/g、(1.96±0.89)μg/g、(3.97±0.82)μg/g、(41.9±3.41)μg/g、(13.2±2.25)μg/g、(8.89±1.29)μg/g。随后,应用硅胶薄层层析,对上述6种大型海藻MAAs提取物进行检测,建立了大型海藻MAAs检测的一种简便方法,可用于大型海藻MAAs的筛选。 A series of single factor and orthogonal experiments were used to establish the extraction process of mycosporine-like amino acids (MAAs) from Laminaria japonica,Undaria pinnatifida,Hizikia fusifarme,Gracilaria arcuata,Gelidium amansii and Gloiopeltis furcata.In this process,MAAs were detected by silica gel thin layer chromatography for the first time.Namely,suitable temperature,solid-liquid ratio and methanol volume fraction of MAAs extraction were 35,30,30,30,30,25 ℃,1:60,1:60,1:60,1:60,1:60,1:50 g/mL,30%,10%,30%,10%,30%,10%,respectively.Under the above conditions,MAAs extracts from 6 kinds of marine macroalgae were prepared with the yield of (39.0±3.80)%,(20.6±2.20)%,(32.8±2.60)%,(5.41±1.20%),(9.87±0.80)% and (16.2±1.40)%,respectively.Furthermore,the results of 250~390 nm wavelength scanning showed that the MAAs extracts of six species of marine macroalgae have absorption near 330 nm.And this means that there was porphyra-334 in them.Further,the contents of porphyra-334 in Laminaria japonica,Undaria pinnatifida,Hizikia fusifarme,Gracilaria arcuata,Gelidium amansii and Gloiopeltis furcata were (1.60±0.69)μg/g,(1.96±0.89)μg/g,(3.97±0.82) μg/g,(41.9±3.41)μg/g,(13.2±2.25)μg/g and (8.89±1.29)μg/g,respectively.This was the first report on the content of porphyra-334 in six kinds of marine macroalgae.Then,silica gel thin layer chromatography was used to detect the MAAs of the six species of marine macroalgae,and a simple method for the detection of MAAs in marine macroalgae was established,which can be used for the screening of MAAs from marine macroalgae.
作者 孙颖颖 杨子轩 周静 韩秀 王炜璟 雷亚婷 SUN Yingying;YANG Zixuan;ZHOU Jing;HAN Xiu;WANG Weijing;LEI Yating(Jiangsu Key Laboratory of Marine Biotechnology,Jiangsu Ocean University,Lianyungang 222005,China;Jiangsu Institute of Marine Resources Development,Lianyungang 222005,China;Co-Innovation Center of Jiangsu Marine Bio-Industry Technology,Lianyungang 222005,China;Lianyungang Quality Technology Comprehensive Inspection and Testing Center,Lianyungang 222006,China)
出处 《食品科技》 CAS 北大核心 2021年第3期205-211,共7页 Food Science and Technology
基金 江苏高校自然科学研究重大项目(19KJA430010) 江苏省“333工程”科研项目(BRA2020263) 连云港市“海燕计划”项目 江苏省优势学科项目 江苏省研究生科研实践创新计划项目(SJCX201297) 江苏海洋大学大学生实践创新训练计划项目。
关键词 大型海藻 类菌孢素氨基酸 Porphyra-334 定性检测 marine macroalgae mycosporine-like amino acids porphyra-334 qualitative detection
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