摘要
目的 探究基因干扰CXCL4对GBC-SD细胞恶性增殖和上皮间质转化的影响.方法 构建shR-NACXCL4载体转染至GBC-SD细胞,将细胞随机分为3组:Control组、shRNA-NC组、CXCL4-shRNA1组.EDU染色检测细胞增殖;流式细胞仪检测细胞凋亡;荧光显微镜观测细胞上皮间质转化形态学变化;West-ern印迹检测Ki67、Survivin、Bax、Bcl-2、E-cadherin和N-cadherin蛋白表达;免疫荧光检测Vimentin含量.结果 与Control组相比,CXCL4-shRNA1组EDU阳性细胞数和Ki67、Survivin、N-cadherin蛋白水平降低,细胞凋亡率、Bax/Bcl-2比值、E-cadherin蛋白水平升高,抑制上皮间质转化,Vimentin含量降低,差异均有统计学意义(P<0.05).结论 基因干扰CXCL4抑制GBC-SD细胞恶性增殖、上皮间充质转化并促进凋亡.
Objective To investigate the effects of CXCL4 gene interference on malignant proliferation and epithelial mesenchymal transformation of GBC-SD cells.Methods shRNA CXCL4 vector was constructed and transfected into GBC-SD cells.The cells were randomly divided into three groups:Control group,shRNA-NC group and CXCL4-shRNA1 group.Cell proliferation was detected by EDU staining.Flow cytometry was used to detect apoptosis.Morphological changes of epithelial mesenchymal transformation were observed by fluorescence microscopy.Western blot was used to detect the expressions of Ki67,Survivin,Bax,Bcl-2,E-cadherin and N-Cadherin.Immunofluorescence was used to detect Vimentin.Results Compared with the Control group,the number of EDU positive cells and the levels of Ki67,Survivin,and N-cadherin in the CXCL4-shRNA1 group were decreased,and the apoptosis rate,Bax/Bcl-2 ratio,E-cadherin level,the inhibition of epithelial stromal transformation,and the level of Vimentin in the CXCL4-shRNA1 group were all significantly decreased(P<0.05).Conclusion The CXCL4 gene interfered inhibits the malignant proliferation,epithelial-mesenchymal transformation and promotes apoptosis of GBC-SD cells.
作者
田野
唐子滨
向海兵
TIAN Ye;TANG Zibin;XIANG Haibing(Department of General Surgery,Armed Police Corps Hospital of Jilin Province,Changchun,130000,China;Department of Orthopedics,Armed Police Corps Hospital of Jilin Province,Changchun,130000,China)
出处
《医学分子生物学杂志》
CAS
2021年第2期104-109,共6页
Journal of Medical Molecular Biology
基金
吉林省卫生厅科研课题(No.2018Z135)。
关键词
胆囊癌
CXC趋化因子配体4
恶性增殖
上皮间质转化
allbladder cancer
chemokine C-X-C motif ligand 4
alignant proliferation
pithelial mesenchymal transformation
