摘要
为从微生物生态学角度探求烤烟根腐病的发生原因并为其防控提供理论依据,利用Illumina MiSeq高通量测序分析了烟草根腐病发病和健康植株根际土壤真菌群落结构的差异,结果表明,烤烟根腐病发生对烤烟根际土壤真菌的丰富度有显著影响,其中健康和患病烟株根际土壤样品中真菌OTU数分别为904个和647个,健康烟株真菌OTU总数为患病烟株的1.40倍,特有OTUs数是患病烟株的2.59倍;患病烟株根际土壤真菌群落多样性水平显著低于健康烟株土壤,患病烟株根际土壤的Shannon、ACE和Chao1指数分别较健康植株降低了29.81%、40.12%和40.61%;同时,患病烟株与健康烟株根际土壤真菌群落的优势物种显著不同,患病烟株根际土壤中烤烟根腐病病原菌茄镰刀菌(F.solani)相对丰度较健康烟株根际土壤增加了303.45%,为健康烟株的4.03倍,且棘孢木霉(Trichoderma asperellum)和青霉菌(Penicillium raperi)的相对丰度亦较健康烟株分别增加了17.62%和50.46%。烟草植株根际土壤中真菌群落结构改变及物种多样性降低是烤烟根腐病发生的重要特征。研究可为该病害的早期预防或生态调控提供科学依据。
In order to explore the causes of tobacco root rot and provide theoretical basis for its prevention and control from the perspective of microbial ecology,Illumina Miseq high-throughput sequencing was used to analyze the effect of tobacco root rot on the fungus community structure and diversity in rhizosphere soil of tobacco plants.The results showed that the occurrence of root rot had a significant influence on the richness of fungi in tobacco rhizosphere soil.Abundance of OTU in the rhizosphere soil at healthy and root rot infected tobacco plants was 904 and 647,respectively.The total number of OTUs of healthy tobacco was 1.40 times of that of root rot infected tobacco,and its unique OTUs was 2.59 times of that of root rot infected tobacco.Analysis of the composition and diversity of fungi showed that Shannon,ACE,and Chao1 index of the rhizosphere soil at root rot infected tobacco were reduced by 29.81%,40.12%,and 40.61%,compared with healthy plants;and the fungus diversity level in rhizosphere soil at root rot infected tobacco was significantly lower than that of the healthy tobacco plants.At the same time,the dominant species of fungus community in rhizosphere soil at root rot infected tobacco and healthy tobacco plants were significantly different.The relative abundance of tobacco root rot pathogen(F.solani)increased by 303.45%compared with the healthy tobacco plants,which was 4.03 times of the healthy tobacco.Meanwhile,the relative abundance of Trichoderma asperellum and Penicillium raperi was increased by 17.62%and 50.46%,compared with the healthy tobacco plants.The above results indicate that the change of fungus community structure and the reduction of fungus species diversity in the rhizosphere soil are important characteristics of tobacco root rot occurrence.This study could provide scientific basis for the early prevention or ecological regulation of tobacco root rot.
作者
郑元仙
杨敏
王继明
许银莲
蔡宪杰
黄飞燕
童文杰
陈小龙
余磊
何元胜
ZHENG Yuanxian;YANG Min;WANG Jiming;XU Yinlian;CAI Xianjie;HUANG Feiyan;TONG Wenjie;CHEN Xiaolong;YU Lei;HE Yuansheng(Lincang Company of Yunnan Tobacco Company,Lincang,Yunnan 677000,China;College of Agronomy,Yunnan Urban Agricultural Engineering&Technological Research Center,Kunming University,Kunming 650214,China;Material Procurement Center,Shanghai Tobacco Group Co.,Ltd,Shanghai 200082,China;Yunnan Academy of Tobacco Science,Kunming 650201,China;China Tobacco Henan Industrial Co.,Ltd.,Zhengzhou 450000,China)
出处
《中国烟草科学》
CSCD
北大核心
2021年第2期50-55,共6页
Chinese Tobacco Science
基金
中国烟草总公司云南省公司科技项目(2018530000241020、2018530000241016、2019530000241011)
上海烟草集团有限责任公司基地单元项目(2020-2021)。
关键词
烤烟
根腐病
根际微生物
群落结构
多样性
tobacco
root rot
rhizosphere microorganism
community structure
diversity
