摘要
目的建立高效液相色谱法测定生活饮用水中六价铬的方法。方法采用InoPacTMAS7为分析柱,InoPacTMAG7为保护柱,探究高效液相色谱法直接测定生活饮用水中的六价铬时,流动相、进样量的选择对结果的影响。结果确定高效液相色谱法测定生活饮用水六价铬的最佳色谱条件,为波长375nm,120mmol/L硫酸铵10%氨水的水溶液为流动相进行洗脱,100μl进样。在10~1000μg/L范围内线性关系良好,相关系数r=0.9999,方法定量限为10μg/L、检出限为3μg/L。在模拟样品中的3个质量浓度下,加标回收率分别为98.9%~101.5%、100.1%~101.8%、99.5%~100.5%,相对标准偏差(RSD,n=7)为0.4%~0.9%。结论建立的方法前处理简单快速、灵敏度高,结果准确可靠,适用于测定生活饮用水中的六价铬含量。
Objective To establish a method for the determination of hexavalent chromium in drinking water by high performance liquid chromatography(HPLC).Methods InoPacTMAS7 column and InoPacTMAG7 column were applied as the analytical column and guard column,respectively.The influence of mobile phase and injection volume on the results of direct determination of hexavalent chromium in drinking water by HPLC was investigated.Results The HPLC parameters were optimized for determination of hexavalent chromium:the wavelength was 375 nm,120 mmoL/L aqueous solution of ammonium sulfate and 10%ammonia water was used as mobile phase for elution,injection volume was set as 100μL.The linear relationship of the method was good in the range of 10-1000μg/L,the correlation coefficient was 0.9999.The limit of quantification was 10μg/L,and the detection limit was 3μg/L.The recovery rates of the spiked samples at three concentrations of simulated samples were 98.9%-101.5%,100.1%-101.8%,99.5%-100.5%,and the relative standard deviation(RSD,n=7)was in a range of 0.4%to 0.9%.Conclusion The established method is simple,rapid and sensitive,and the results are accurate and reliable,which is suitable for the determination of hexavalent chromium in drinking water.
作者
凌映茹
张昊
吉文亮
LING Ying-ru;ZHANG Hao;JI Wen-liang(Jiangsu Provincial Center for Disease Control and Prevention,Jiangsu Nanjing 210009,China)
出处
《江苏预防医学》
CAS
2021年第2期127-129,共3页
Jiangsu Journal of Preventive Medicine
基金
江苏省医学重点学科(ZDXKA2016008)。
关键词
高效液相色谱法
生活饮用水
六价铬
High performance liquid chromatography
Drinking water
Hexavalent chromium
