摘要
目的:研究骨形态发生蛋白4(BMP4)对乳腺癌细胞MCF-7增殖、迁移和侵袭能力的影响。方法:将重组慢病毒Lv-BMP4及干扰慢病毒Lv-shBMP4感染MCF-7细胞,构建MCF-7/Lv-BMP4过表达组及MCF-7/LvshBMP4干扰实验组,同时设空白组(Blank)、慢病毒空载对照组(Lv-NC)、过表达实验组(Lv-BMP4)、干扰试验组(Lv-shBMP4)。RT-PCR及ELISA技术检测MCF-7细胞中BMP4过表达及干扰情况;CCK-8法检测MCF-7细胞的增殖能力;划痕愈合和Transwell实验检测MCF-7细胞的迁移能力和侵袭能力。结果:BMP4过表达的乳腺癌细胞株MCF-7/Lv-BMP4及BMP4低表达的干扰细胞株MCF-7/Lv-shBMP4构建成功;BMP4过表达组的BMP4蛋白表达水平较对照组显著升高,而Lv-shBMP4组的BMP4蛋白表达水平较对照组显著降低,表明BMP4过表达的乳腺癌细胞株MCF-7/Lv-BMP4及BMP4低表达的干扰细胞株MCF-7/Lv-shBMP4构建成功。BMP4过表达组的细胞增殖较对照组受到明显抑制,增殖活力较对照组明显降低;48 h后Lv-BMP4组划痕愈合能力较对照组显著降低,而Lv-shBMP4组划痕愈合能力较对照组明显升高;48 h后Lv-BMP4组MCF-7细胞穿膜数显著低于对照组,而Lv-shBMP4组MCF-7细胞穿膜数显著高于对照组。结论:BMP4可抑制乳腺癌MCF-7细胞的增殖和迁移。
Objective:To study the effect of bone morphogenetic protein 4(BMP4)on the proliferation,migration and invasion of breast cancer cell line MCF-7 in vitro.Methods:MCF-7 cells were infected with lentivirus Lv-BMP4 and lentivirus Lv-shBMP4 to construct MCF-7/Lv-BMP4 overexpression group and MCF-7/Lv-shBMP4 interference group.There were 4 groups in this experiment:Blank group,Lv-NC group,Lv-BMP4 group and Lv-shBMP4 group.RT-PCR and ELISA were used to detect BMP4 overexpression and interference in MCF-7 cells.CCk-8 assay was used to detect the proliferation of MCF-7 cells.Scratch healing and Transwell were used to detect the migration and invasion ability of MCF-7 cells.Results:RT-PCR and ELISA showed that the BMP4 over-expressed breast cancer cell lines MCF-7/Lv-BMP4 and the endogenous BMP4 down-regulated cell lines MCF-7/Lv-shBMP4 were successfully constructed.The CCK-8 results showed that the cell proliferation in BMP4 over-expressed group was significantly inhibited but the cell proliferation significantly low in MCF-7/Lv-shBMP4 group when compared with the control group.Wound-healing assay found that the scratch healing rate in Lv-BMP4 group was significantly lower than that in the control group after 48 h,while the scratch healing rate in Lv-shBMP4 group was significantly higher than that in the control group.Transwell migration assay showed that MCF-7 cells in Lv-BMP4 group getting through a few films after 48 h was significantly lower than that in the control group,while the migrated number of MCF-7 cells in Lv-shBMP4 group was significantly higher.Conclusion:BMP4 can inhibit the proliferation and migration of breast cancer MCF-7 cells in vitro.
作者
李海云
杨东光
洪秀丽
李金波
王辉
Li Haiyun;Yang Dongguang;Hong Xiuli;Li Jinbo;Wang Hui(Department of General Surgery,Zhuozhou City Hospital Zhuozhou 072750,China)
出处
《解剖学杂志》
CAS
2021年第2期123-128,共6页
Chinese Journal of Anatomy
基金
保定市科技计划项目(17ZF300)。
关键词
骨形态发生蛋白4
慢病毒
乳腺癌
增殖
迁移
bone morphogenetic protein 4
lentivirus
breast cancer
proliferation
migration
