摘要
目的通过室间质量评价(EQA)了解上海地区临床实验室真菌培养鉴定及体外药物敏感性试验的开展情况及检测质量。方法2019年EQA共发放10份样本(冻干菌株)进行真菌培养鉴定,其中4份要求同步进行体外药物敏感性试验。通过反馈结果对真菌鉴定符合率和体外药物敏感性试验的开展情况进行统计分析。结果共收到75份有效回报结果,其中有45份进行了真菌体外药物敏感性试验。10株菌株(涵盖2个属9个种)中有4株鉴定结果符合率为100%,鉴定结果有误的菌株分别为葡萄牙念珠菌、季也蒙念珠菌、近平滑念珠菌、白念珠菌、罗伦隐球菌和新生隐球菌。2019年第1次EQA有93.3%(70/75)的实验室结果完全正确,6.7%(5/75)的实验室出现错误结果但总成绩合格;第2次EQA有94.7%(71/75)的实验室结果完全正确,4.0%(3/75)的实验室出现错误结果但总成绩合格,1.3%(1/75)的实验室成绩不合格。结论上海地区临床实验室真菌培养鉴定总体检测能力和准确率较高,开展真菌体外药物敏感性试验项目的临床实验室数量有限。质量控制对于保证检测结果的准确性具有十分重要的作用。
Objective To study the development and determination quality of fungal culture identification and in vitro drug sensitivity test in Shanghai through external quality assessment(EQA).Methods In 2019,EQA issued a total of 10 samples for culture and identification,of which 4 samples required further drug sensitivity test.According to the feedback results,the coincidence rate of fungal identification and the development of in vitro drug sensitivity test were analyzed.Results A total of 75 effective returns were received,45 of which were tested for fungal drug sensitivity.The 4 of 10 strains(including 2 genera and 9 species)were identified with 100%coincidence rate,and the strains with wrong identification results were Candida lusitaniae,Candida guilliermondii,Candida parapsilosis,Candida albicans,Cryptococcus laurentii and Cryptococcus neoformans.In the first EQA in 2019,93.3%(70/75)of the laboratory results were completely correct,6.7%(5/75)of the laboratories had wrong results,but the total score was qualified.In the second EQA,94.7%(71/75)of the laboratory results were completely correct,4.0%(3/75)of the laboratories had wrong results,but the total scores were qualified,and 1.3%(1/75)of the laboratories were unqualified.Conclusions The overall determination ability and accuracy rate of fungal culture and identification in clinical laboratories in Shanghai are relatively high.The number of clinical laboratories carrying out fungal drug sensitivity test is limited.Quality control in clinical laboratories is essential to assure the accuracy of results.
作者
陈蓉
张敏敏
夏启航
钱诚凯
崔琳
刘学杰
徐蓉
王庆忠
葛平
黄维纲
王敬华
CHEN Rong;ZHANG Minmin;XIA Qihang;QIAN Chengkai;CUI Lin;LIU Xuejie;XU Rong;WANG Qingzhong;GE Ping;HUANG Weigang;WANG Jinghua(Shanghai Center for Clinical Laboratory,Shanghai 200126,China)
出处
《检验医学》
CAS
2021年第4期441-443,共3页
Laboratory Medicine
基金
河南省科技厅横向联合项目(181200211900)。
关键词
真菌
培养鉴定
体外药物敏感性试验
室间质量评价
质量控制
Fungus
Culture identification
In vitro drug sensitivity test
External quality assessment
Quality control
