摘要
目的:探究不同炮制方法对黄柏药效的影响。方法:选择Balb/c小鼠为实验动物,将其随机分为蜜制组、盐制组、酒制组、生品组与模型组,每组10只,建立溃疡性结肠炎模型,蜜制组、盐制组、酒制组、生品组给予对应药液灌胃干预,比较各组疾病活动指数(DAI)、结肠长度、结肠大体形态损伤指数、组织学评分及结肠组织炎症介质[肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)、白细胞介素-17(IL-17)]、氧化应激指标[超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)]差异。结果:其中蜜制组、酒制组DAI及组织学评分均明显低于盐制组、生品组,结肠组织TNF-α、IL-17水平均明显低于盐制组、生品组,结肠组织IL-10水平均明显高于盐制组、生品组,差异有统计学意义(P<0.05);蜜制组、盐制组、酒制组结肠长度及结肠组织SOD、GSH-Px水平均明显高于生品组,结肠大体形态损伤指数及结肠组织MDA水平均明显低于生品组,差异有统计学意义(P<0.05)。结论:不同方法炮制黄柏均可增强对溃疡性结肠炎的药效,尤以蜜制、酒制黄柏增强效果较为突出,可为其炮制工艺提供参考。
Objective:To explore the effects of different processing methods on pharmaceutical effects of Cortex Phellodendri.Methods:Balb/c mice were selected as experimental animals.And were randomly divided into a ulcerative colitis group,a honey processing group,a salt processing group,a wine processing group,a crude herb group respectively,with 10 mice in each group.The disease activity index(DAI),colon length,colon gross morphological damage index,histological score and colonic cytokines[tumor necrosis factor-α(TNF-α),interleukin-10(IL-10),interleukin-17(IL-17)]and oxidative stress indicators[superoxide dismutase(SOD),malondialdehyde(MDA),glutathione peroxidase(GSH-px)]were compared between the 2 groups.Results:The DAI and histological score in honey processing group and wine processing group were significantly lower than those in salt processing group and crude herb group.The levels of TNF-αand IL-17 in colon tissues were significantly lower than those in salt processing group and crude herb group while the level of IL-10 in colon tissues was significantly higher than that in salt processing group and crude herb group(P<0.05).The colon length and levels of SOD and GSH-px in colon tissues in honey processing group,salt processing group and wine processing group were significantly higher than those in crude herb group,and the colon gross morphological damage index and the MDA level in colon tissues were significantly lower than those in crude herb group(P<0.05).Conclusion:Different processing methods of Cortex Phellodendri can enhance the pharmaceutical effects of ulcerative colitis,especially the honey-processing and wine-processing Cortex Phellodendri have more prominent enhancement effect,which can provide reference for processing technology.
作者
赵洪超
关书博
王丹
ZHAO Hongchao;GUAN Shubo;WANG Dan(The Second Hospital of Traditional Chinese Medicine,Shenyang 110101,China)
出处
《世界中医药》
CAS
2021年第4期608-611,共4页
World Chinese Medicine
基金
辽宁省科学技术计划项目(2019-ZD-0973)。
关键词
黄柏
炮制
药效
溃疡性结肠炎
小鼠
疾病活动指数
结肠长度
结肠大体形态损伤指数
组织学评分
炎症介质
氧化应激指标
Cortex phellodendri
Processing
Pharmaceutical effects
Ulcerative colitis
Mice
Disease activity index
Colon length
Colon gross morphological damage index
Histological score
Cytokines
Oxidative stress indicators
