摘要
目的:介绍一种简易、多用途的小鼠固定装置的制作和使用方法。方法:选用废弃的50 m L离心管,按设计方案进行切割、组装制成简易小鼠固定装置。选取60只小鼠,随机分为2组,对照组采用传统固定方法,研究组则采用本装置固定,先后对小鼠进行尾静脉注射、断尾采血、膝关节腔内注射,观察比较两组操作时间和成功率等指标。结果:单个50 mL离心管仅重约12 g,制作时间约15 min。对照组和研究组小鼠尾静脉注射时间分别是(32.10±1.97)s和(30.27±2.20)s,成功率均为100%,断尾采血时间分别是(30.10±1.73)s和(28.67±1.55)s,成功率也均为100%,两组在操作时间上差异均具有统计学意义(P<0.01),而在成功率方面均无统计学差异(P>0.05)。两组膝关节腔内注射时间分别是(40.77±2.73)s和(38.73±2.16)s,成功率分别为83.33%和95.00%,两组在操作时间及成功率方面差异均具有统计学意义(P<0.05)。结论:该小鼠固定装置具有材料易得、制作简单、携带方便、用途多样等优点,为实验小鼠的固定提供了一种新的安全、实用的方法。
Objective:This study aims to introduce the manufacture and use instruction for a simple and multifunctional device for mouse fixation. Methods:Discarded 50 mL centrifuge tubes were collected and processed into the fixation device according to the predesigned program. A total of 60 mice were randomly and equally assigned into two groups and fixed with traditional methods(control group)and this fixation device(study group),respectively. Tail vein injections or tail-clamping blood sampling or intra-articular injections were performed. The procedure durations and success rate were recorded. Results:A single 50 ml centrifuge tube weighed about 12 g and mere 15 minutes were taken to manufact the fixation device. The procedure durations of tail vein injection,tail-clamping blood sampling and intra-articular injections in control and study groups were(32.10±1.97)s and(30.27±2.20)s,(30.10±1.73)s and(28.67±1.55)s,and(40.77±2.73)s and(38.73±2.16)s,respectively. The data between two groups were significantly different(P<0.01). The success rates of the three procedures in control group study group were 100% and 100 %,100% and 100%,and 83.33% and 95.00%,respectively. There were significant differences in procedure duration and success rate between two groups(P<0.05). Conclusion:The mouse fixation device possesses the advantages of easily available materials,simple manufacture,convenient transportation and multiple functions. It provides a new safe and reliable choice for mouse fixation in the experiment and is worthy to be promoted.
作者
秦豪男
刘星婧
丁其瑞
马成
魏易凡
任永信
QIN Haonan;LIU Xingjing;DING Qirui;MA Cheng;WEI Yifan;REN Yongxin(Department of Orthopedics,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029;Department of Endocrinology,Zhongda Hospital Affiliated to Southeast University,Nanjing 210009,China)
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2021年第2期280-285,共6页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金面上项目(81572149)。
关键词
小鼠
固定装置
尾静脉注射
断尾采血
关节腔内注射
mouse
fixation device
tail vein injection
tail-clipping blood sampling
intra-articular injection
