摘要
目的采用自制的固定器控制SD大鼠,并用改进的采血方法对其尾静脉进行连续采血,根据相关指标评价其效果。方法 SD大鼠24只在乙醚麻醉下,采用改进的一次性静脉输液针进行尾静脉采血并补充生理盐水,每日1次,连续13 d。观察采血成功率和大鼠存活率。结果 24只SD大鼠连续12 d采血成功;1只SD大鼠在第13天死亡,原因为乙醚麻醉过量,只采到该只大鼠0.2 m L血液。采血成功率为99.68%,SD大鼠存活率为95.83%。结论使用改良后的一次性静脉输液针,实施SD大鼠尾静脉采血并补充生理盐水的方法安全有效、灵活方便,适用于连续时间点采集SD大鼠静脉血的实验研究。
Objective To continuously draw blood from caudal vein of SD rats which controlled by the homemade holder with an improved method,and the effects of this method was evaluated according to the relevant indicators. Methods The 24 SD rats should be drew the blood from the caudal vein by the improved disposable infusion needle and supplied physiological saline at the condition of ether inhalation anesthesia and once a day until to the thirteenth day,and then the times of draw blood and the number of survival rats only to calculate the success rate and survival rate of rats were record. Results In the 12 days,the tail vein blood of 24 SD rats were successfully collected.But a rat died of excessive ether inhalation anesthesia on the thirteenth day. The success rate was 99. 68% and the rat survival rate was95. 83%. Conclusion The method that applying an improved disposable infusion needle and supplying physiological saline is safe and effective,flexible and convenient,which is suitable for experimental study that needs to draw blood from caudal vein of the SD rat at the continuous time.
出处
《局解手术学杂志》
2015年第5期505-507,共3页
Journal of Regional Anatomy and Operative Surgery
基金
国家自然科学基金(81302921)
关键词
SD大鼠
连续
尾静脉
采血
SD rat
continuous
caudal vein
blood sampling
