摘要
目的:探讨MIN-6β细胞经人工合成丝素-Ⅳ型胶原支架三维立体培养后用于移植治疗Ⅰ型糖尿病的新方法。方法 :冷冻干燥法制备丝素-Ⅳ型胶原支架并检测,将MIN-6β细胞种植于丝素-Ⅳ型胶原支架中三维培养,观察细胞定植及细胞活力。将种植有MIN-6β细胞复合培养的丝素-Ⅳ型胶原支架移植入Ⅰ型糖尿病小鼠腹腔,监测对比其血糖变化情况和胰岛素的表达。结果:丝素-Ⅳ型胶原支架为不规则片层样与多孔相结合的结构,各支架三维培养组效果均明显优于传统二维平面培养组,差异存在统计学意义(P<0.05),各支架复合培养组移植入糖尿病动物体内后的降血糖效果与作用时间均要优于无支架组,差异存在统计学意义(P<0.05)。移植物中MIN-6β细胞胞浆内存在有特异性棕黄色受染区域,证实存在胰岛素表达。结论:丝素-Ⅳ型胶原支架有良好的生物相容性且有利于MIN-6β细胞的生长定植及功能表达。
Objective：To investigate the new method of curing the type Ⅰ Diabetes by cultivating and trans-planting the composite artificial scaffold of MIN-6 β cells. Methods：Silk-collagen type Ⅳ scaffold was prepared by the method of freeze-drying. We implant MIN-6 β cells on the silk-collagen type Ⅳ scaffold and then observe the distribution situation of the growing cells and the vitality differences between those cells that growing on the ordinary plane and the composite scaffold. Reaction difference between those cells was compared. After transplantation,the change of the blood glucose level was monitored and the function of cell secretion was tested.Results：More β structure appeared after the silk-collagen type Ⅳ scaffold cross-linked by the methanol. The vitality of those cells cultivated on the scaffold was obviously better than those on the ordinary plane（P 0.05）. Both groups had a good stimulation reaction on both low high concentration glucose,and the scaffold groups had a better cell secretory function than that of the ordinary plane groups（P 0.05）. MIN-6 β cells were dyed to brown and the cells still had a nice insulin secretory function.Conclusion：MIN-6 β cells which are three-dimensionally cultivated by the silk-collagen type IV scaffold have better vitality and relatively long-lasting insulin secretory function.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第2期176-181,共6页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金(81471801)
关键词
丝素蛋白
Ⅳ型胶原
糖尿病
胰岛素
silk protein
silk-collagen type Ⅳ
diabetes
insulin
