摘要
目的探索小鼠胚胎胰腺组织分离技术，并对其形态学及内分泌功能进行鉴定。方法胚胎期（embryonic，E）11．5～16．5dC57BL／6小鼠胚胎胰腺组织体视显微镜下进行分离。利用常规HE染色、免疫组织化学及免疫荧光实验对分离的E12．5d、E14．5d及E16．5d胚胎胰腺组织进行组织学鉴定；糖刺激实验观察胚胎胰腺组织的内分泌功能。结果〉E12．5d小鼠胚胎胰腺组织可较易完整分离。≤E12．5d小鼠胚胎胰腺组织的形态和颜色均难以与周围组织分辨，需根据其与毗邻脏器的关系仔细辨别。分离的早期胚胎胰腺组织已有胰岛素表达，随着胚胎发育，胚胎胰腺组织胰岛素表达强度逐渐增加。结论小鼠胚胎发育和解剖学知识掌握，可提高胚胎胰腺组织分离技术，保证分离获得完整的胚胎胰腺组织，为胚胎胰腺组织移植研究奠定基础。
Objective To explore the isolation technique of embryonic pancreatic tissues in mice and to identify the isolated embryonic pancreatic tissue by morphology and endocrine function test. Methods Pancreat- ic tissues from embryonic（E） 11.5-16.5 d C57BL/6 mouse embryos were surgically isolated under the stereo- microscope. El2. 5 d, El4. 5 d, El6. 5 d embryonic pancreas tissues were identified by conventional HE stai- ning, immunohistochemistry and immunofluorescence. The endocrine functions of embryonic pancreatic tissues were measured by glucose stimulation test. Results Embryonic pancreatic tissues of mice over embryonic 12. 5 days can be easily isolated. Embryonic pancreatic tissues of mice younger than embryonic 12. 5 days were hard to achieve and these pancreatic tissues could only be isolated according to the relationship with the adjacent organs. Histological and ELISA examinations showed that early embryonic pancreatic tissues could express and secrete in- sulin and the insulin level was gradually increased with developmental time. Conclusion A good command of the knowledge of the development and anatomy of mouse embryo help improve isolation of embryonic pancreas, which provides therapeutic potential for embryonic pancreatic tissue transplantation.
出处
《中华内分泌外科杂志》
CAS
2012年第6期367-370,共4页
Chinese Journal of Endocrine Surgery
基金
国家自然科学基金（81170721）
关键词
胚胎
器官原基
分离
免疫组织化学
免疫荧光
小鼠
Embryo
Organ primordia
Isolation
Immunohistoehemistry
Immunofiuoreseence
Mice
