摘要
目的探究小檗碱对流感病毒感染小鼠免疫功能及炎症反应的调节作用。方法选取60只小鼠分为正常对照组、流感病毒组和小檗碱组,20只/组;除正常对照组外各组使用流感病毒滴鼻制成流感病毒小鼠模型;使用1 mg/ml小檗碱给小鼠灌胃0.4 ml/d。检测肺泡灌洗液中白细胞总数及各类白细胞数;苏木精-伊红(HE)染色观察小鼠肺组织病理改变情况;采用流式法检测辅助性T细胞(Th)1/Th2及Th17/调节性T细胞(Treg)比例;检测血清中白细胞介素(IL)-6、IL-4和肿瘤坏死因子(TNF)-α水平;使用蛋白质免疫印迹法检测视黄酸诱导基因蛋白-1(RIG-1)、线粒体抗病毒信号蛋白(MAVS)及核因子(NF)-κB蛋白表达情况。结果HE染色显示,流感病毒组肺组织炎性细胞增多、肺泡结构被破坏,小檗碱组小鼠炎性细胞数目减少。相比正常对照组,流感病毒组小鼠肺泡灌洗液中白细胞总数及各类白细胞数、Th1/Th2、Th17/Treg、IL-6、TNF-α、RIG-1蛋白、MAVS蛋白及NF-κB蛋白水平均升高,IL-4水平降低(P<0.05);相比流感病毒组,小檗碱组小鼠肺泡灌洗液中白细胞总数及各类白细胞数、Th1/Th2、Th17/Treg、IL-6、TNF-α、RIG-1蛋白、MAVS蛋白及NF-κB蛋白水平均降低,IL-4水平升高(P<0.05)。结论小檗碱可以通过影响RLH信号传导通路的活化,调节T淋巴细胞亚群比例,同时抑制炎症反应来实现抗流感病毒的作用。
OBJECTIVE To explore the regulatory effects of berberine on immune function and inflammation response of mice with influenza virus infection.METHODS A total of 60 mice were divided into the control group,the influenza virus group and the berberine group,with 20 mice in each group.All the groups were given nasal drops of influenza virus to prepare influenza virus mice models except the control group.The mice were administered intragastrically with 1 mg/ml of berberine(0.4 ml/d).The counts of total white blood cells(WBC)and various types of WBC in bronchoalveolar lavage fluid(BALF)were detected,the pathological changes of lung tissues were observed by HE staining,the proportions of Th1/Th2 and Th17/Treg were detected by flow cytometry,the levels of serum interleukin-6(IL-6),IL-4 and tumor necrosis factor-α(TNF-α)were detected,and the expression of retinoic acid inducible gene protein-1(RIG-1),mitochondrial antiviral signal protein(MAVS)and nuclear factor(NF)-κB was detected with the use of western blot(WB)hybridization.RESULTS The HE staining showed that inflammation cells in lung tissues of the influenza virus group were significantly increased,and alveolar structures were destroyed,and the inflammatory cells counts of the berberine group were remarkably decreased.The counts of total WBC and various types of WBC in BALF,Th1/Th2,Th17/Treg,IL-6 level,TNF-αlevel,RIG-1 level,MAVS level and NF-κB level of the influenza virus group were higher significantly than those of the control group,while the IL-4 level of the influenza virus group was significantly lower than that of the control group(P<0.05).The counts of total WBC and various types of WBC in BALF,Th1/Th2,Th17/Treg,IL-6 level,TNF-αlevel,RIG-1 level,MAVS level and NF-κB level of the berberine group were lower than those of the influenza virus group,while the IL-4 level of the berberine group was significantly higher than that of the influenza virus group(P<0.05).CONCLUSION Berberine can regulate the proportions of T lymphocyte subsets by affecting the acti
作者
黄莺
呼少娜
王莹
HUANG Ying;HU Shao-na;WANG Ying(Wuhan Children's Hospital,Wuhan,Hubei 430000,China)
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2021年第6期801-805,共5页
Chinese Journal of Nosocomiology
基金
湖北省卫生计生委基金资助项目(WJ2017M197)。
关键词
流感病毒
小檗碱
免疫功能
炎症反应
Influenza virus
Berberine
Immune function
Inflammatory response
