摘要
目的探讨疱疹性咽峡炎患儿血清微小RNA-21(miR-21)表达,并分析其与辅助性T淋巴细胞17(Th17)/调节性T淋巴细胞(Treg)、转化生长因子-β1(TGF-β1)、白细胞介素(IL)-17、IL-6、IL-2和IL-10的关系。方法选取2018年11月至2020年4月在该院就诊的68例疱疹性咽峡炎患儿作为试验组;选取同期在该院体检的63例健康儿童作为对照组。采用实时荧光定量PCR(qPCR)检测血清中miR-21的表达情况;采用双抗体夹心酶联免疫吸附测定(ELISA)检测血清中TGF-β1、IL-17、IL-2、IL-6和IL-10水平;采用流式细胞仪检测外周血单个核细胞中的Th17、Treg表达情况,计算Th17/Treg;采用Pearson相关分析miR-21与Th17/Treg及相关炎症因子的相关性。结果试验组miR-21的相对表达水平、Th17百分率、Th17/Treg高于对照组,Treg百分率低于对照组,差异有统计学意义(P<0.05);试验组TGF-β1、IL-17和IL-6水平高于对照组,IL-2和IL-10水平低于对照组,差异有统计学意义(P<0.05);疱疹性咽峡炎患儿血清中miR-21相对表达水平与Th17/Treg、TGF-β1、IL-17及IL-6水平均呈正相关(P<0.05),与IL-2、IL-10水平均呈负相关(P<0.05)。结论miR-21相对表达水平及Th17/Treg在疱疹性咽峡炎患儿血清中均升高,且二者呈正相关,提示miR-21可能通过影响炎性反应,从而影响疱疹性咽峡炎的疾病进展。
Objective To investigate the expression of serum microRNA-21(miR-21)in children with herpetic angina,and to analyze its relationship with helper T lymphocyte 17(Th17)/regulatory T lymphocyte(Treg),transforming growth factor-β1(TGF-β1),interleukin(IL)-17,IL-6,IL-2 and IL-10.Methods A total of 68 children with herpetic angina who were treated in this hospital from November 2018 to April 2020 were selected as the experimental group;63 healthy children who received physical examination in the hospital during the same period were selected as the control group.Real time fluorescent quantitative PCR(qPCR)was used to detect the expression of miR-21 in serum;double antibody sandwich enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of TGF-β1,IL-17,IL-2,IL-6 and IL-10 in serum;flow cytometry was used to detect the expression situation of Th17 and Treg in peripheral blood mononuclear cell,and the ratio of Th17/Treg was calculated;Pearson correlation was used to analyze the correlation between miR-21 and Th17/Treg and related inflammatory factors.Results The relative expression level of miR-21,Th17 percentage,and Th17/Treg of the experimental group were higher than those of the control group,and the Treg percentage was lower than that of the control group,the differences were statistically significant(P<0.05);the levels of TGF-β1,IL-17 and IL-6 in the experimental group were higher than those in the control group,while the levels of IL-2 and IL-10 in the experimental group were lower than those in the control group,the differences were statistically significant(P<0.05);the relative expression level of miR-21 in the serum of children with herpangina were positively correlated with Th17/Treg,TGF-β1,IL-17 and IL-6(P<0.05),and negatively correlated with IL-2 and IL-10(P<0.05).Conclusion The relative expression level of miR-21 and Th17/Treg in the serum of children with herpangina are increased,and they are positively correlated,suggesting that miR-21 may affect the disease progression of herpangina by af
作者
董德明
翟敏
廖廷彦
DONG Deming;ZHAI Min;LIAO Tingyan(Department of Pediatrics,Zaoyang First People′s Hospital,Zaoyang,Hubei 441200,China)
出处
《国际检验医学杂志》
CAS
2021年第7期793-796,共4页
International Journal of Laboratory Medicine
基金
湖北省卫生健康委员会科研项目(WJ2018MB117)。
