摘要
目的探讨2021年深圳地区疱疹性咽峡炎的流行病学和病原学特征,为疱疹性咽峡炎的防控提供科学参考。方法收集2021年深圳地区哨点医院210例疱疹性咽峡炎病例的基本信息及其咽拭子、肛拭子和粪便样本408份。采用荧光定量PCR进行肠道病毒检测,采用半巢式PCR结合测序分析进行肠道病毒分型。结果210例疱疹性咽峡炎患者年龄集中在5岁以下,平均年龄(2.7±1.7)岁,疾病流行高峰在春季,患者的主要临床表现为发热和口腔疱疹。咽拭子和肛拭子样本的阳性率一致且一致性较高(Kappa=0.785,P=1.000)。粪便样本的检出阳性率高于咽拭子,差异有统计学意义(χ^(2)=12.000,P<0.001)。总肠道病毒阳性率为84.8%,共检测出7种肠道病毒,包括柯萨奇病毒A2型(CV-A2)、CV-A4、CV-A5、CV-A6、CV-A10、CV-A16和CV-B3,其中CV-A4(38.8%)和CV-A10(35.4%)占比较高。不同月份的病原分布不同,差异有统计学意义(χ^(2)=115.344,P<0.001)。感染CV-A16患者出现发热症状的比例低于其他病原体,差异有统计学意义(χ^(2)=11.005,P<0.05)。感染CV-A2患者发热体温高于其他病原体,差异有统计学意义(F=2.658,P<0.05)。结论2021年深圳地区疱疹性咽峡炎发病人群以5岁以下为主,春季为流行高峰,感染肠道病毒主要为CV-A4和CV-A10,需加强柯萨奇病毒的监测。
Objective To investigate the epidemiological and etiological characteristics of herpangina in Shenzhen in 2021,and provide scientific reference for the prevention and control of herpangina.Methods Two hundred and ten cases of herpangina from sentinel hospitals were collected in Shenzhen city in 2021,and 408 samples of throat swabs,anal swabs and stool samples were included.Fluorescence quantitative PCR was used for enterovirus detection,and semi-nested PCR combined with sequencing analysis were used for enterovirus typing.Results Among 210 herpangina patients,most patients were under 5 years old and the average age was(2.7±1.7)years old.The peak of herpangina epidemic was in spring,and the main clinical manifestations of patients were fever and oral herpes.The positive rates of throat swab samples and anal swab samples were consistent and highly concordant(Kappa=0.785,P=1.000),while the positive rates of stool samples were higher than throat swabs(χ^(2)=12.000,P<0.001).The total enterovirus positive rate was 84.8%and seven enteroviruses were detected,including coxsackie virus A2(CV-A2),CV-A4,CV-A5,CV-A6,CV-A10,CV-A16 and CV-B3;of which,CV-A4(38.8%)and CV-A10(35.4%)accounted for a higher proportion.The distribution of enteroviruses in different months was different(χ^(2)=115.344,P<0.001),and patients infected with CV-A16 had lower rates of fever than others(χ^(2)=11.005,P<0.05)and patients infected with CV-A2 had higher body temperature than others(F=2.658,P<0.05).Conclusions The majority of herpangina patients in Shenzhen city in 2021 was mainly under five years old,and the epidemic peak was in spring.The main pathogens were CV-A4 and CV-A10,and comprehensive surveillance of coxsackievirus should be strengthened.
作者
潘倩瑜
杨洪
何雅青
陈龙
张海龙
姚相杰
张仁利
孟君
PAN Qianyu;YANG Hong;HE Yaqing;CHEN Long;ZHANG Hailong;YAO Xiangjie;ZHANG Renli;MENG Jun(School of Public Health,Southern Medical University,Guangzhou,Guangdong 510515,China;Department of Pathogenic and Microbiology,Shenzhen Center for Disease Control and Prevention,Shenzhen,Guangdong 518055,China)
出处
《热带医学杂志》
CAS
2023年第7期900-903,913,共5页
Journal of Tropical Medicine
基金
深圳市科技创新可持续发展专项(KCXFZ20201221173404012)
广东省自然科学基金(2019A1515010394)
国家十三五科技重大专项(2018ZX10713-001-009)
