摘要
目的探讨1,8-桉叶油素(1,8-Cineole)通过调节自噬来改善高糖(HG)诱导人主动脉内皮细胞(HAECs)损伤的保护作用。方法采用HG作用HAECs 60 h,不同剂量的1,8-Cineole进行干预,运用噻唑蓝(MTT)法检测细胞活力,乳酸脱氢酶(LDH)检测细胞毒性,Western blot法检测Beclin1、LC3-Ⅱ/Ⅰ、p62、caspase-3和caspase-9的蛋白表达,及使用自噬抑制剂氯喹(CQ)作用HAECs后,检测Beclin1、LC3-Ⅱ/Ⅰ、p62、caspase-3和caspase-9的表达情况。结果1,8-Cineole提高细胞活力,减少LDH含量,激活自噬和抑制细胞凋亡。与空白组比较,CQ组的Beclin1、LC3-Ⅱ/Ⅰ、p62、caspase-3和caspase-9表达增加,同时观察到CQ+HG+CH组与CQ+HG组比较时,以上蛋白差异均无统计学意义。结论1,8-Cineole对高糖诱导HAECs损伤具有保护作用,其作用与改善自噬流相关。
Aim To investigate the protective effect of 1,8-Cineole on the injury of human aortic endothelial cells(HAECs)induced by high glucose(HG)via regulating autophagy.Methods Cells were incubated with different doses of 1,8-Cineole followed by exposing to HG for 60 h,and MTT assay was used to analyse cell viability,lactate dehydrogenase(LDH)was used to detect cytotoxicity,and Western blot was used to detect Beclin1,LC3-Ⅱ/Ⅰ,p62,caspase-3 and caspase-9 expressions.Autophagy inhibitor(chloroquine,CQ)was treated on HAECs,and the expressions of Beclin1,LC3-Ⅱ/Ⅰ,p62,caspase-3 and caspase-9 were measured by Western blot.Results 1,8-Cineole increased cell viability,reduced the content of LDH,activated autophagy and inhibited apoptosis.Compared with control group,the expression of Beclin1,LC3-Ⅱ/Ⅰ,p62,caspase-3 and caspase-9 in CQ group increased.Simultaneously,the expression of above-mentioned between CQ+HG group and CQ+HG+CH group.Conclusions 1,8-Cineole has protective effect on the injury of HAECs induced by high glucose,and its effect is related to improving autophagy flux.
作者
宋晓妹
杨红
龙秋双
方官琴
高歌
甘诗泉
陈妍
沈祥春
SONG Xiao-mei;YANG Hong;LONG Qiu-shuang;FANG Guan-qin;GAO Ge;GAN Shi-quan;CHEN Yan;SHEN Xiang-chun(College of Pharmacy,Guizhou Medical University,Guiyang 550025,China;the High Educational Key Lab of Guizhou Province for Natural Medicinal Pharmacology and Druggability,Guiyang 550025,China;Dept of Pharmacy,Maternal and Child Health Hospital of Guiyang,Guiyang 550003,China)
出处
《中国药理学通报》
CAS
CSCD
北大核心
2021年第4期472-477,共6页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81760725)
国家自然科学基金喀斯特中心项目(No U1812403-4-4)
贵州省科技基金重点项目(黔科合基础[2020]1Z069)
贵州省高层次创新型人才百层次人才(黔科合人才[2015]4029号)
贵州省药学国际科技合作基地(黔科合平台人才[2017]5802)
贵州省科学技术基金2019年度基础研究计划项目(黔科合基础[2019]1279号)
贵州省卫生健康委科学技术基金(No gzwjkj2019-1-185)。
