摘要
目的:探讨神气汤对慢性疲劳综合征(CFS)大鼠免疫功能的影响及其作用机制。方法:将40只健康雌性SD大鼠随机分为正常组、模型组、帕罗西汀组和神气汤组,每组各10只。除正常组外,其余各组制备CFS模型,模型制备成功后模型组不给药、帕罗西汀组和神气汤组灌胃相应的药物。检测各组大鼠旷场实验评分、免疫器官指数及血清免疫球蛋白G(IgG)、免疫球蛋白A(IgA)的表达水平。结果:正常组大鼠在整个实验过程中未见明显异常;模型组大鼠对外界的适应能力减弱,如懒动、烦躁易怒等;神气汤组、帕罗西汀组大鼠的皮毛脱落状况较给药前好转,大便成形、情绪正常。与正常组比较,模型组大鼠水平运动次数、垂直运动次数、胸腺指数、脾脏指数及IgA、IgG均减少(P<0.05),中央格停留时间增加(P<0.05)。与模型组比较,神气汤组、帕罗西汀组水平运动次数、垂直运动次数、胸腺指数、脾脏指数及IgA、IgG均增加(P<0.05),中央格停留时间减少(P<0.05)。与帕罗西汀组比较,神气汤组IgG、IgA增加(P<0.05)。结论:神气汤能够提高CFS大鼠的免疫器官指数,上调IgG、IgA的表达,改善大鼠的免疫水平,治疗CFS。
Objective:To discuss the effect of Shenqi tang on immune functions in rats with chronic fatigue syndrome(CFS)and its mechanism.Methods:A total of 40 healthy male SD rats were randomly divided into the normal group,the model group,the paroxetine group and the Shenqi tang group,10 rats in each group.Except for the normal group,CFS models were established in the other groups.After successful establishment,the model group was not given drugs,the paroxetine group and the Shenqi tang group were respectively treated with the corresponding medicine by gavage.The scores of open field test,the immune organ indexes and the expression levels of immunoglobulin G(IgG)and immunoglobulin A(IgA)in serum in rats were detected.Results:During experiment,there was no significant abnormality in the normal group;in the model group,the adaptability to external was weakened,such as lethargy,dysphoria and irascibility;in the Shenqi tang group and the paroxetine group,the fur shedding state was improved when compared with that before drug administration,with forming stool and normal emotions.Compared with those in the normal group,the times of horizontal motion and vertical motion,thymus indexes,spleen indexes and IgA and IgG in the model group were decreased(P<0.05),and the time of staying in center was increased(P<0.05).Compared with those in the model group,the times of horizontal motion and vertical motion,thymus indexes,spleen indexes and IgA and IgG in the Shenqi tang group and the paroxetine group were increased(P<0.05),and the time of staying in center was decreased(P<0.05).Compared with those in the paroxetine group,the IgG and IgA in the Shenqi tang group were increased(P<0.05).Conclusion:Shenqi tang can improve immune organ indexes in CFS rats,up-regulate the expressions of IgG and IgA,improve immune levels in rats and treat CFS.
作者
李立华
张兆辉
LI Lihua;ZHANG Zhaohui
出处
《新中医》
CAS
2021年第4期6-9,共4页
New Chinese Medicine
基金
北京中医医院顺义医院两院合作项目(SYYJ-201703)。
关键词
慢性疲劳综合征
神气汤
免疫因子
旷场实验
动物实验
大鼠
Chronic fatigue syndrome
Shenqi tang
Immune factors
Open field test
Animal experiment
Rats
