摘要
目的分析单采血小板保存期内活化及相关功能和代谢情况变化。方法选择2019年单采血小板的献血者50例,分别留取保存d0(采集当天4 h内)、d1、d2、d3、d4的单采血小板标本各10例,共5组。检测各组采集血小板活化指标PAC-1、CD62P和用血栓弹力图评估功能,测定血小板计数及相关参数,测定生化指标pH、葡萄糖、乳酸及乳酸脱氢酶反映代谢情况,进行统计分析。结果单采血小板在保存期内不同时期的参数检验结果,血小板活化指标CD62P(%)和PAC-1(%)多组间差异有显著性(P<0.05),保存d4血小板晚期活化指标CD62P明显增加(P<0.05)。TEG参数R值(min)、K值(min)、ANG(min)、MA(mm)多组间差异有显著性(P<0.05),保存d1MA值明显增加(P<0.05)。Plt及相关指标多组间差异没有显著性(P>0.05)。血小板生化指标方差分析显示多组间差异有显著性(P<0.05),保存d4的血小板PH值和葡萄糖最低,乳酸和乳酸脱氢酶最高。结论单采血小板在保存期内不同时期出现活化、功能和代谢变化等情况,血小板发生一定程度的贮存损伤。
Objective To analyze the changes of activation, function and metabolism of apheresis platelets during storage.Methods Five groups of apheresis platelets(n=10 per group), stored up to 5 days at 20~24℃ with agitation,were sampledat day0(4 hours within collection), 1,2,3 and 4, respectively to assess the activation indexes as PAC-1 and CD62 P and platelet function was evaluated by thromboelastogram.The platelet counts and related parameters were determined, and the biochemical indexes such as pH, glucose, lactic acid and lactate dehydrogenase reflecting metabolism were measured and statistically analyzed.Results The CD62 P(%) and PAC-1(%) of apheresis platelets with different storage duration showed significant differences(P<0.05),and CD62 P(%) increased significantly on 4 thday of storage(P<0.05). There were significant differences in TEG parameters as R(min), K(min), ANG(min)and MA(mm),and MA value increased significantly after1-daystorage(P<0.05). There was no significant difference in Plt and related indexes among the groups(P>0.05). Significant differences were notable in platelet biochemical indexesby groups using variance analysis(P<0.05). The pH value and glucose were the lowest in platelets with 4-day storage, while lactic acid and lactate dehydrogenase were the highest.Conclusion The storagelesion of apheresis platelets occurs as the activation, function and metabolism of platelets developed as the storage prolonged.
作者
姚勇
张欢欢
孙振超
吴运萍
唐梦怡
YAO Yong;ZHNAG Huanhuan;SUN Zhenchao;WU Yunping;TANG Meng(1.Lianyungang Red Cross Central Blood Station,Lianyungang 222001,China;Department of Laboratory,The First People's Hospital of Lianyungang;Department of Blood Transfusion.Lianyungang Second People's Hospital.)
出处
《中国输血杂志》
CAS
2021年第2期120-123,共4页
Chinese Journal of Blood Transfusion
基金
江苏省输血协会英科新创科研基金(Js2018004)。
关键词
单采血小板
保存期
血小板活化
血小板功能
血小板代谢
apheresis platelets
storage
platelet activation
platelet function
platelet metabolism
