摘要
本试验利用体外批次培养法,通过测定培养各时间点的瘤胃发酵参数并结合多项组合效应值(MFAEI),研究向底物中添加不同剂量的诺丽果原粉对绒山羊体外瘤胃发酵功能的影响,为绒山羊生产中新型替抗产品的开发及诺丽果资源的合理利用提供理论支撑。试验以2周岁内蒙古阿尔巴斯白绒山羊为瘤胃液供体动物,采用完全随机区组试验设计,分别向发酵底物中添加0、1.0%、2.0%、3.5%和5.0%的诺丽果原粉,并分别培养3、6、9、12和24 h。结果表明:不同添加剂量的诺丽果原粉均显著降低了体外瘤胃发酵pH、氨态氮(NH 3-N)浓度、原虫数量和乙酸/丙酸(P<0.05),显著提高了菌体蛋白(BCP)浓度、总挥发性脂肪酸(TVFA)浓度和MFAEI(P<0.05),且添加剂量为3.5%时可较好地促进瘤胃发酵功能。由此可见,添加诺丽果原粉可促进绒山羊的体外瘤胃发酵功能,并能降低原虫数量,且均在剂添加量为3.5%时效果较好。
The objective of this study was to evaluate the effect of different levels of noni fruit raw powder(Morinda citrigolia L.)on in vitro rumen fermentation function of cashmere goats through in vitro batch culture method and by determining the parameters of rumen fermentation in different culture time combined with multiple forages associative effect indicator(MFAEI).The results of this study could provide theoretical support for the development of new alternative antibiotic products in the production of cashmere goats and the rational utilization of noni fruit resources.The 2-year-old Inner Mongolia Albas white cashmere goats was used as rumen fluid donor animals.Five doses of noni fruit raw powder(0,1.0%,2.0%,3.5%and 5.0%)was added in the substrate cultured for 3,6,9,12 and 24 h with randomized complete block design.The results showed that different addition doses of noni fruit raw powder significantly decreased the pH,ammonia nitrogen(NH 3-N)concentration,protozoa number and acetate acid/propionic acid of rumen fermentation in vitro(P<0.05),and significantly increased the bacterial protein(BCP)concentration,total volatile fatty acid(TVFA)concentration and MFAEI(P<0.05),and the addition dose of 3.5%could better promote rumen fermentation function.It is concluded that the addition of noni fruit raw powder can promote the in vitro rumen fermentation of cashmere goats,decrease the protozoa number,and the above effects are better when the addition dose is 3.5%.
作者
张清月
梁晓帅
李胤豪
马国强
郭晓宇
赵艳丽
闫素梅
ZHANG Qingyue;LIANG Xiaoshuai;LI Yinhao;MA Guoqiang;GUO Xiaoyu;ZHAO Yanli;YAN Sumei(Inner Mongolia Autonomous Region Key Laboratory of Animal Nutrition and Feed Science,Collage of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China)
出处
《动物营养学报》
CAS
CSCD
北大核心
2021年第2期869-876,共8页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
“十二五”农村领域国家科技计划课题(2012BAD12B09-3)
自治区草原英才工程产业创新人才团队计划。
关键词
诺丽果原粉
绒山羊
体外瘤胃发酵
产气量
多项组合效应值
noni fruit raw powder
cashmere goats
in vitro rumen fermentation
gas production
multiple forages associative effect indicator
