摘要
为探究不同极性的溶剂提取对刺梨物质含量变化及抗氧化活性的影响,分别以水、甲醇、60%乙醇、无水乙醇、正丁醇和乙酸乙酯为提取溶剂,通过测定提取物中抗坏血酸、总多酚、总黄酮、单宁和总三萜含量,采用体外抗氧化方法对刺梨不同溶剂提取物进行抗氧化活性测定,并采用抗氧化活性综合(antioxidant potency composite,APC)指数法进行比较分析。结果表明:60%乙醇提取物中总多酚、总黄酮、单宁和总三萜含量最高;水提取物中抗坏血酸含量最高;乙酸乙酯提取物中总多酚、总黄酮、抗坏血酸和总三萜含量最低;正丁醇提取物中单宁含量最低。对抗氧化活性而言,60%乙醇提取物清除DPPH自由基与ABTS+自由基能力和还原力最强;甲醇提取物的羟基自由基清除率最高;乙酸乙酯提取物清除超氧阴离子自由基的能力最强。APC指数显示,6种溶剂提取物的抗氧化能力由强到弱依次为60%乙醇提取物>甲醇提取物>水提取物>无水乙醇提取物>乙酸乙酯提取物>正丁醇提取物。由于不同活性成分之间存在协同或拮抗作用,且不同活性成分在不同溶剂中的溶解性不同,因此不同溶剂提取对刺梨活性物质含量和抗氧化活性影响较大。
Rosa roxburghii Tratt was extracted with different solvents including water,methanol,60%ethanol,anhydrous ethanol,n-butanol,and ethyl acetate.The content of vitamin C(VC),total polyphenols,total flavo-noids,tannins,and total triterpenoids in the extracts was determined.The antioxidant activity antioxidant potency composite(APC)in vitro was evaluated for different extracts.The results showed that the content of total polyph-enols,total flavonoids,tannins,and total triterpenoids was the highest in the 60%ethanol extract and that of VC in the water extract.The content of total flavonoids,VC,and total triterpenoids was the lowest in ethyl acetate extract and that of tannins in n-butanol extract.The 60%ethanol extract showed the strongest DPPH and ABTS radical scavenging activities and reducing power.The methanol extract and ethyl acetate extract showed the strongest hydroxyl and superoxide anion radical scavenging activities,respectively.According to APC,the antioxidant activity was in a descending order of 60%ethanol extract>methanol extract>water extract>anhydrous ethanol extract>ethyl acetate extract>n-butanol extract.Because of the synergistic or antagonistic effects between different active components and the varied solubility of different active components in different solvents,different solvents have great impacts on the active component content and antioxidant activity of R.roxburghii extract.
作者
穆兴燕
吴胜
郭银萍
刘含
徐菁
刘晓燕
MU Xingyan;WU Sheng;GUO Yinping;LIU Han;XU Jing;LIU Xiaoyan(Food and Pharmaceutical Engineering Institute,Guiyang University,Guiyang 550005,Guizhou,China;Guizhou Engineering Research Center for Fruit Processing,Guiyang 550005,Guizhou,China)
出处
《食品研究与开发》
CAS
北大核心
2023年第17期68-75,共8页
Food Research and Development
关键词
刺梨
提取物
生物活性成分
活性物质
抗氧化
Rosa roxburghii Tratt
extract
bioactive ingredients
active components
antioxidant
