摘要
目的探讨武汉市甲、乙型流感病毒的流行特征,通过比较巢式PCR和胶体金法检测对流感病毒的检出情况,评价后者能否为临床流感样患者的流感初筛和诊断提供快速有效的方法。方法采集2017—2019年武汉市流感样患者的鼻咽拭子,进行胶体金法检测,分析该地区甲、乙型流感病毒的流行病学特征;同时选取其中600份样本,进行巢式PCR检测复核结果。结果2017—2019年,各年份中甲型流感、乙型流感、混合感染检出率比较,差异均有统计学意义(P<0.05);流感呈季节性;流感患者中18岁以下的人群所占比例较高(P<0.05);巢式PCR和胶体金法的一致性检验显示,Kappa=0.40,二者一致性一般。结论胶体金法检测简便快速,能较好地满足对于临床标本初筛的要求,但其检测的灵敏度和特异度仍待提高。
Objective To investigate the epidemiological characteristics of influenza in Wuhan,and compare the consistency between the rapid antigen detection and nested PCR to verify whether the sensitivity and specificity of the former provide an effective method for the diagnosis of clinical influenza-like patients.Methods Collected the nasopharyngeal swabs from patients with flu-like symptoms from 2017 to 2019,and then colloidal gold method was used to test the samples.The epidemiological characteristics of influenza A and B viruses in the area were analyzed,meanwhile 600 samples were selected to be rechecked by nested PCR.Results From 2017 to 2019,there were statistically significant differences in the detection rates of influenza A,B and mixed infection in each year(P<0.05);influenza was seasonal;the proportion of influenza patients under 18 years old was higher than the other age groups(P<0.05);the consistency test of nested PCR and colloidal gold method showed that Kappa=0.40,the consistency was general.Conclusion Colloidal gold method is convenient and rapid,which can meet the requirements of clinical samples screening,but its sensitivity and specificity still need to be improved.
作者
盛鄂湘
陈良君
段勇威
李一荣
SHENG E′xiang;CHEN Liangjun;DUAN Yongwei;LI Yirong(Department of Clinical Laboratory,Zhongnan Hospital of Wuhan University,Hubei 430061,China)
出处
《国际检验医学杂志》
CAS
2021年第4期444-446,452,共4页
International Journal of Laboratory Medicine
基金
武汉大学自主科研项目(2042018kf0058)。
