摘要
目的:探讨流感病毒细胞分离培养阳性检出的主要影响因素。方法:收集流感样病例的咽拭子中PCR检测为阳性的标本作为实验的研究对象,运用MDCK细胞培养法分离培养流感病毒,分析对比病例,运用logistic回归法分析影响病毒分离阳性率的因素。结果:PCR阳性标本中分离阳性率为60.2%。研究发病至采样时间与收样至接种时间等因素带给分离病毒结果的影响,证明间隔时间越短,病毒分离阳性率就越高(P<0.05)。结论:减少发病到采集样本时间间隔及收集样本到接种时间间隔,就可以提升分离流感病毒的阳性率。
Objective:To explore the main factors affecting the positive detection of influenza virus isolation and culture. Methods:the specimens of influenza like swabs collected from hospital, were selected as the subjects of the test as positive. The MDCK cell culture method was used to isolate and culture influenza virus. The contrast cases were analyzed, and the logistic regression analysis was used to analyze the impact of the virus. The factor of positive rate. Results:the positive rate of PCR positive specimens was 60.2%. The effects of sampling time, sampling time and inoculation time on the results of the virus isolation were studied. The shorter the interval time, the higher the positive rate of the virus isolation(P<0.05). Conclusion:reducing the time interval between onset and collection of samples and collecting samples to vaccination intervals can enhance the positive rate of influenza virus isolation.
作者
董悦
DONG Yue(Chaoyang City Center for Disease Control and prevention,Chaoyang 122000,Liaoning)
出处
《安徽卫生职业技术学院学报》
2018年第6期105-106,共2页
Journal of Anhui Health Vocational & Technical College
关键词
流感病毒
分离培养
阳性率
影响因素
Influenza virus
Isolation and culture
Positive rate
Influencing factors
