摘要
目的探讨水孔蛋白-5(AQP-5)在不同机械通气时间致大鼠急性肺损伤(ALI)肺组织中的表达。方法将30只SD大鼠,按照机械通气时间不同,完全随机分为对照组、0.5 h组、1.0 h组、1.5 h组和2.0 h组,每组6只。通过肺组织大体标本图、肺系数、HE染色,观察肺组织水肿及损伤程度;采用病理切片染色评分法评估各组肺组织中AQP-5表达量。结果 5组肺系数、AQP-5染色强度评分和AQP-5阳性细胞占比评分比较,经方差分析,差异有统计学意义(P <0.05)。肺系数与AQP-5染色强度评分呈负相关(rs=-0.925,P <0.05);肺系数与AQP-5染色阳性细胞占比评分之间呈负相关(rs=-0.951,P <0.05)。结论随着机械通气时间延长,肺水肿程度逐渐加重,肺组织中AQP-5表达量逐渐减少,表明AQP-5参与机械通气致肺水肿的发生、发展。
Objective To investigate the expression of Aquaporin-5(AQP5)in the lung tissues of rats with acute lung injury(ALI)induced by differen tmechanical ventilation times.Methods 30 male SD rats were selected and randomly divided into 5 groups according to different hours of mechanical ventilation,namely the control group,0.5 h group,1.0 h group,1.5 h group,and 2.0 h group,with 6 rats in each group.Observation indicators,such as lung tissue edema and injury degree by gross specimen of lung tissue,lung coefficient,and HE staining,were detected.Immunohistochemistry was used to evaluate the expression of AQP5 in lung tissue of each group with pathological section staining and scoring.Results Five groups of lung coefficient,AQP5 staining intensity score,and AQP5 positive cell proportion score were compared respectively.After analysis of variance,the differences were statistically significant(F=376.693,207.522 and 216.311,all P<0.05).Lung coefficient was negatively correlated with AQP5 staining intensity score(rs=-0.925,P<0.05);lung coefficient was negatively correlated with AQP5 staining positive cell proportion score(rs=-0.951,P<0.05).Conclusion With the extension of mechanical ventilation time,the degree of pulmonary edema gradually increased and the expression of AQP5 in lung tissue gradually decreased,which indicated AQP5 is involved in the occurrence and development of pulmonary edema induced by mechanical ventilation.
作者
陈美羽
赵毓静
张新日
Mei-yu Chen;Yu-jing Zhao;Xin-ri Zhang(Department of Pulmonary and Critical Care Medicine,The First Hospital of Shanxi Medical University,Shanxi,Taiyuan 030001,China)
出处
《中国现代医学杂志》
CAS
北大核心
2021年第3期1-5,共5页
China Journal of Modern Medicine
基金
山西省重点研发计划项目(No:201803D31093)
中央引导地方科技发展专项资金项目(No:YDZX20191400002737)。
关键词
急性肺损伤
肺水肿
水孔蛋白-5
机械通气
acute lung injury
pulmonary edema
aquaporin 5
respiration,artificial
