摘要
目的评价机械通气相关性肺损伤大鼠肺组织水通道蛋白5（AQP5）表达的变化。方法健康雄性SPF级SD大鼠70只，周龄7周，体重200—250g，随机分为7组（n=10），对照组（A组）仅切开气管保留自主呼吸；不同通气时间小潮气量组（B1组和B2组）Vτ 7ml／kg，分别通气2h或4h；不同通气时间中潮气量组（C1组和C2组）VT20ml／kg，分别通气2h或4h；不同通气时间大潮气量组（D1组和D2组）Vτ 40ml／kg，分别通气2h或4h。A组在气管切开即刻，其余各组在机械通气结束时处死大鼠，开胸取肺组织行病理损伤评分，测定AQP5蛋白及其mRNA表达，计算肺组织湿／干重比值（W／D比值），收集肺泡灌洗液，计数中性粒细胞（PMN）。结果与A组比较，C1组、C2组、D1组、D2组AQP5蛋白及其mRNA表达下调，W／D比值、PMN计数和病理损伤评分升高（P〈0．05）。与B1组比较，C1组和D1组AQP5蛋白及其mRNA表达下调，W／D比值、PMN计数和病理损伤评分升高（P〈0．05）。与B2组比较，C2组和D2组AQP5蛋白及其mRNA表达下调，W／D比值、PMN计数和病理损伤评分升高（P〈0．05）。与C1组比较，D1组AQP5蛋白及其mRNA表达下调，W／D比值、PMN计数和病理损伤评分升高（P〈0．05）。与C2组比较，D2组AQP5蛋白及其mRNA表达下调，W／D比值、PMN计数和病理损伤评分升高（P〈0．05）。结论AQP5表达下调参与了机械通气诱发大鼠肺水肿的发生。
Objective To investigate the expression of aquapofin 5 （AQP5） in lung tissue in a rat model of ventilator-induced lung injury （VILI）. Methods Seventy pathogen free male SD rats were anesthetized with intraperitoneal 10% choral hydrate 3.5 ml/kg, tracheostomized and mechanically ventilated. The animals were randomly assigned into 7 groups （n = 10 each） according to the tidal volume （Vτ）, respiratory rate （RR） and duration of mechanical ventilation： group A spontaneous breathing; group B1, B2 （Vτ 7 ml/kg, RR 50 bpm, I： E 1：3, duration 2 h, 4 h）; group C1, C2（Vτ 20 ml/kg, RR50 bpm, I：E 1：3, duration 2 h, 4 h） and group D1, D2 （Vτ 40 ml/kg, RR 50 bpm, I：E 1：3, duration 2 h, 4 h）. The animals were killed at the end of mechanical ventilation. The lungs were removed for microscopic examination, and determination of wet to dry lung weight ratio （W/D ratio）, PMN counts in broncho-alveolar lung lavage fluid （BALF） and expression of AQP5 mRNA and protein in lung tissue. Results The expression of AQP5 mRNA and protein was significantly down-regulated while W/D ratio and PMN counts in BALF were significantly increased in group C and D than in group A and B. Conclusion The down-regulation of AQP5 expression in lung tissue may be involved in the development of lung edema during mechanical ventilation.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2010年第2期234-237,共4页
Chinese Journal of Anesthesiology
