摘要
目的观察不同处理方式艾燃烧生成物对载脂蛋白E基因敲除(ApoE-/-)小鼠血清炎症因子及斑块稳定性的影响。方法40只雄性8周龄ApoE-/-小鼠随机均分为5组:艾烟组、滤过艾烟组、艾绒挥发组、艾叶精油组、模型组。8只同龄雄性C57BL/6小鼠为空白对照。艾烟组置于遮光率为2%的艾烟环境;滤过艾烟组置于艾烟滤过后的环境;艾绒挥发组置于150℃加热艾绒挥发环境;艾叶精油组置于精油雾化环境。干预14周后,ELISA测定血清γ干扰素(IFN-γ)、白细胞介素-10(IL-10)、基质金属蛋白酶9(MMP-9)、基质金属蛋白酶抑制剂1(TIMP-1)含量,HE染色观察主动脉病理改变。结果与模型组相比,艾烟组与滤过艾烟组小鼠血清INF-γ、MMP-9显著降低(P>0.05),IL-10、TIMP-1显著升高(P>0.05),主动脉病变显著减轻,两组间无显著差异(P<0.05)。结论艾烟与滤过艾烟可抑制血管内炎症反应及稳定斑块,有效缓解动脉粥样硬化病变。
Objective:To observe the effects of moxa combustion products processed under different conditions on serum inflammatory factor and plaque stability in ApoE-/-mice.Methods:A total of 40 male ApoE-/-mice(8-week-old)were randomly divided into 5 groups:moxa smoke group,filtered moxa smoke group,volatile components of moxa floss group,essential oil of artemisia argyi group and model group.Additionally,8 male C57 BL/6 mice(8-week-old)were used as a control group.The mice in moxa smoke group were exposed to shading rate 2%concentration of moxa smoke,the mice in filtered moxa smoke group were exposed to filtered moxa smoke environment,the mice in volatile components of moxa floss group were exposed to 150℃moxa floss heating environment,the mice in essential oil of artemisia argyi group were exposed to artemisia argyi essential oil atomization environment.All interventions were carried out for 14 weeks.The contents of INF-γ,IL-10,MMP-9 and TIMP-1 in serum were tested with ELISA,the pathological changes of aorta were observed by HE staining.Results:Compared with the model group,the INF-γ and MMP-9 in the moxa smoke group and filtered moxa smoke group decreased significantly(P>0.05),while IL-10 and TIMP-1 increased significantly(P>0.05);the pathological changes of aorta in the moxa smoke group and filtered moxa smoke group were significantly alleviated,and there was no significant difference between the two groups(P<0.05).Conclusion:Moxa smoke and filtered moxa smoke can inhibit inflammatory reaction in mice,stabilize plaque and effectively alleviate atherosclerotic lesions.
作者
黄跃平
姚琴
欧阳夏荔
刘津艺
惠鑫
王昊
和蕊
张瑞
赵百孝
Huang Yueping;Yao Qin;Ouyang Xiali;Liu Jinyi;Hui Xin;Wang Hao;He Rui;Zhang Rui;Zhao Baixiao(Beijing University of Chinese Medicine,Beijing 102488,China)
出处
《中国中医急症》
2021年第1期41-43,62,共4页
Journal of Emergency in Traditional Chinese Medicine
基金
国家自然科学基金项目(81874503)。
关键词
艾燃烧生成物
动脉粥样硬化
炎症因子
斑块稳定性
小鼠
Moxa combustion products
Atherosclerosis
Inflammatory factors
Plaque stability
Mice
