摘要
近年来煤炭加工给环境造成的污染问题越来越受到重视。利用土壤微生物修复技术治理污染土壤得到了极大关注。利用甲苯或苯甲酸作为唯一碳源和能源物质作研究对象,在硝酸盐作电子受体还原条件下,厌氧富集焦化污染土壤菌液,分析菌液中甲苯或苯甲酸降解过程以及硝酸盐浓度的变化,同时通过高通量测序技术对厌氧反硝化菌液中甲苯降解菌和苯甲酸降解菌的菌群结构多样性进行比较分析。实验证明,甲苯或苯甲酸与硝酸盐反应的物质的量比分别为1∶8.7和1∶5.9,这个数据和理论计量学比率在合理程度上可认为相近。在120 d厌氧脱氮富集焦化污染土壤菌液过程中,焦化土壤中的主要菌门为Proteobacteria,反硝化菌液中降解甲苯的优势门为Firmicutes,而降解苯甲酸菌液中的优势门为Proteobacteria.α-proteobacteria、γ-proteobacteria、Bacilli是降解甲苯土壤中的优势纲,而降解苯甲酸土壤中优势纲为β-proteobacteria.Firmicutes门中Exiguobacterium是甲苯降解菌液的优势菌属,而在苯甲酸土壤菌液中Azoarcus为优势菌属。
With a greater amount of attention paid to the pollution of the environment caused by coal processing,methods using soil microorganism repairing to improve coking contaminated soil becomes more and more significant.The biodegradation of toluene or benzoate and the nitrate-reducing process were analyzed in coking contaminated soil by the addition of toluene or benzoate as the sole carbon source as well as energy source in enrichment solution under nitrate-reducing,then the structural diversity of anaerobic denitrification microbial community of degradation toluene or benzoate was compared by high throughput sequencing.The molar ratio of toluene or benzoate to nitrate was 1∶8.7 or 1∶5.9,respectively,a reasonable approximation of the theoretical stoichiometric ratios.After 120 days of enrichment,the main bacteria in the coking soil is Proteobacteria,and the advantage phylum of phenol degradation in soil enrichment under the condition of nitrate reduction is Firmicutes,while for benzoate it is Proteobacteria.α-,andγ-proteobacteria,Bacilli is the dominant class in toluene-degrading bacteria,and the dominant class in the degradation of benzoate isβ-proteobacteria.The results show that Exiguobacterium is the predominant genus of toluene-degrading bacteria,while Azoarcus is the dominant species in benzoate.
作者
王建伟
李亚男
张国凯
饶竹
王国英
王迪
岳秀萍
WANG Jianwei;LI Yanan;ZHANG Guokai;RAO Zhu;WANG Guoying;WANG Di;YUE Xiuping(College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China;Chinasea Group Environmental Protection Engineering Co., Ltd., Taiyuan 030012, China;National Research Center for Geoanalysis, Beijing 100037, China)
出处
《太原理工大学学报》
CAS
北大核心
2021年第1期45-52,共8页
Journal of Taiyuan University of Technology
基金
山西省基础研究计划项目资助(2015021119)
自然资源部生态地球化学重点实验室开放基金资助(No.ZSDHJJ201804)
山西省2019年度研究生教育改革研究课题项目。
关键词
焦化污染土壤
甲苯
苯甲酸
硝酸根还原
高通量测序
coking contaminated soil
toluene
benzoate
nitrate-reducing
high throughput sequencing
