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酶联免疫法筛查人类免疫缺陷病毒抗体在艾滋病诊断中的应用

The Application of Screening Human Immunodeficiency Virus Antibody by Enzymelinked Immunosorbent Assay in Diagnosing AIDS
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摘要 目的探讨酶联免疫法筛查人类免疫缺陷病毒(HIV)抗体在艾滋病诊断中的应用效果。方法选择2019年1—12月于医院接受HIV抗体筛查的1000例艾滋病疑似患者作为研究对象,所有患者均行酶联免疫法与金标斑点法检测,以初筛及印证试验联合诊断结果为金标准,比较酶联免疫法与金标斑点法的诊断准确度、灵敏度、特异度。结果酶联免疫法的诊断准确度为99.90%,高于金标斑点法的98.90%,差异有统计学意义(P<0.05);酶联免疫法的诊断灵敏度及特异度分别为100.00%、99.90%,高于金标斑点法的75.00%、99.00%,差异有统计学意义(P<0.05)。结论在艾滋病诊断中应用酶联免疫法筛查HIV抗体具有较高的应用价值,可为临床诊断提供有效依据。 Objective The application effect of screening human immunodeficiency virus(HIV)antibody by enzyme-linked immunosorbent assay(ELISA)in diagnosing AIDS was explored.Methods A total of 1000 suspected AIDS patients who have received HIV antibody screening in the hospital from January to December in 2019 were selected as the subjects in this study.All patients were tested by(ELISA)and dot immunogold assay.The results of preliminary screening combined with verified tests were defined as the gold standards and then the diagnostic accuracy,sensitivity and specificity were compared between ELISA and dot immunogold assay.Results The diagnostic accuracy of ELISA(99.90%)was higher than that of dot immunogold assay(98.90%),and the difference was statically significant(P<0.05).The diagnostic sensitivity and specificity of ELISA(100.00%,99.80%)were both higher than those of dot immunogold assay(75.00%,99.00%),and the differences were statistically significant(P<0.05).Conclusion The application value of ELISA in screening HIV antibody for diagnosing AIDS was relatively high,thus could providing effective references for clinical diagnosing.
作者 冯燕兰 Feng Yanlan(Clinical Laboratory,Maoming Martenal and Child Health Hospital,Maoming Guangdong 525000,China)
出处 《医疗装备》 2021年第1期44-45,共2页 Medical Equipment
关键词 酶联免疫法 艾滋病 人类免疫缺陷病毒抗体 诊断价值 Enzyme-linked immunosorbent assay AIDS Human immunodeficiency virus antibody Diagnostic value
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