摘要
目的探讨成年病毒性肺炎患者T淋巴细胞亚群的水平变化及临床意义。方法选取该院2017年3月至2019年4月收治的62例病毒性肺炎患者为观察组,另选取同期该院65例健康体检者为对照组。采集观察组患者肺泡灌洗液,使用酶联免疫吸附试验(ELISA)检测病毒IgM抗体;采集两组静脉血,采用流式细胞术检测T淋巴细胞亚群,采用ELISA检测炎症因子[白细胞介素-6(IL-6)、C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)]水平。分析观察组的病毒感染类型;比较两组T淋巴细胞亚群及炎症因子水平。结果观察组单一病毒感染45例,占72.58%,混合感染17例,占27.42%。观察组CD3^+、CD4^+、CD4^+/CD8^+水平明显低于对照组,CD8^+水平明显高于对照组,差异均有统计学意义(P<0.05)。观察组IL-6、CRP、TNF-α水平明显高于对照组,差异均有统计学意义(P<0.05)。结论成年病毒性肺炎患者以单一病毒感染为主,患者T淋巴细胞亚群功能紊乱,机体炎性反应水平升高。
Objective To explore the level changes and clinical significance of T lymphocyte subsets of adult patients with viral pneumonia.Methods A total of 62 patients with viral pneumonia admitted to the hospital from March 2017 to April 2019 were selected as the observation group.In addition,65 healthy people who underwent physical examination in the hospital during the same period were selected as the control group.Collected the alveolar lavage fluid of the patients in the observation group,and used the enzyme-linked immunosorbent assay(ELISA)to detect the virus IgM antibodies.Collected venous blood of the two groups,used flow cytometry to detect T lymphocyte subsets,used ELISA to detect the levels of inflammatory factors[interleukin-6(IL-6),C-reactive protein(CRP),tumor necrosis factor-α(TNF-α)].Analyzed the type of virus infection in the observation group.Compared the T lymphocyte subsets and inflammatory factors levels of the two groups.Results In the observation group,there were 45 cases of single virus infection,accounting for 72.58%,and 17 cases of mixed infection,accounting for 27.42%.The CD3^+,CD4^+,CD4^+/CD8^+levels in the observation group were significantly lower than those in the control group,and CD8^+level was significantly higher than that in the control group,the differences were statistically significant(P<0.05).The levels of IL-6,CRP and TNF-αin the observation group were significantly higher than those in the control group,and the differences were statistically significant(P<0.05).Conclusion Adult patients with viral pneumonia are mainly infected by single virus.The function of T lymphocyte subsets of the patients are disordered and the level of inflammatory reaction is increased.
作者
张间霞
庄健海
吕微风
邓秋萍
叶桂样
ZHANG Jianxia;ZHUANG Jianhai;LYU Weifeng;DENG Qiuping;YE Guiyang(Department of Clinical Laboratory,Foshan Traditional Chinese Medicine Hospital,Foshan,Guangdong 528000,China)
出处
《检验医学与临床》
CAS
2020年第24期3601-3603,共3页
Laboratory Medicine and Clinic
关键词
病毒性肺炎
T淋巴细胞亚群
炎症因子
viral pneumonia
T lymphocyte subsets
inflammatory factors
