摘要
目的检测病毒性肺炎患者BALF中T细胞亚群,探讨病毒性肺炎患者免疫功能的变化。方法采集病毒性肺炎组及对照组BALF,用ELISA法检测RSV、Flu、ADV、PIV和CMV特异性Ig M,碱性磷酸酶-抗碱性磷酸酶(APAAP)桥联酶标法检测T细胞亚群(CD3、CD4、CD8和CD4/CD8)。结果 55例病毒肺炎患者中,单独病毒感染41例,占大部分,其中Flu 12例居首位,依次为RSV 9例、PIV 8例、ADV 6例和CMV 6例;混合感染14例,依次为Flu+PIV 5例、Flu+RSV 3例、ADV+PIV 3例、ADV+Flu 2例和PIV+RSV 1例。病毒性肺炎组T细胞亚群CD3和CD4降低,CD8增高,CD4/CD8比值减小,与对照组比较(P<0.05)差异有统计学意义。结论病毒感染是成人肺炎的主要病原之一,单独病毒感染为主,混合感染为辅,病毒病原谱具有地区差异。病毒性肺炎患者的CD3、CD4、CD8和CD4/CD8存在一定的变化规律,及早进行病原学及免疫功能检测,有利于及时采取相应的治疗措施。
【Objective】To test the T cells subsets in a viral pneumonia patients BALF and discuss the immune function of patients with viral pneumonia of change. 【Methods】Acquisition of viral pneumonia group and control group BALF, RSV, Flu,ADV, PIV, CMV specific Ig M, were detected by ELISA, alkaline phosphatase-resistance to alkaline phosphatase（APAAP） was used to test T cell subsets（ CD3, CD4, CD8, CD4/CD8）.【Results】Of 55 patients with virus pneumonia, alone the infected virus were 41 cases, of 41 cases, 12 cases of Flu stands first, 9 for RSV, 8cases of PIV, 6 cases of ADV, 6 cases of CMV 6; Mixed infected is 14, Flu＋PIV 5, Flu＋RSV 3, ADV＋PIV3, ADV＋Flu 2, PIV＋RSV i1. Viral pneumonia T cell subsets CD3, CD4 was reduced, CD8 increased, CD4/CD8 ratio decreased compared with controls（P〈0.05）. 【Conclusions】The virus infection is one of the main causes of adult＇s pneumonia, alone virus infection is the first, mixed infection is the second, the virus causes have difference in the region. CD3, CD4, CD8, CD4/CD8 of viral pneumonia patients have certain variation. Early diagnosis of etiology and immunological function is useful to take appropriate treatment.
出处
《中国现代医学杂志》
CAS
北大核心
2015年第7期41-43,共3页
China Journal of Modern Medicine
基金
河南科技大学大学生训练计划(SRTP)资助项目(No:2011036)
关键词
病毒性肺炎
肺泡灌洗液
T淋巴细胞亚群
virus pneumonia
bronchoalveolar lavage fluid（BALF）
T lymphocyte subsets
