摘要
目的:观察葫芦夜交汤对卒中后抑郁(post stroke depression,PSD)模型大鼠行为及神经递质的影响。方法:将80只健康雄性SD大鼠随机分为对照组、模型组、氟西汀组和葫芦夜交汤组,每组20只。除对照组外,其余各组采用线栓大脑中动脉法制备脑卒中大鼠模型,再采用随机给予多种慢性刺激的方法制备PSD模型,对照组大鼠不予任何干预。对照组和模型组大鼠均给予等剂量生理盐水灌胃,氟西汀组予以氟西汀2 mg·kg-1灌胃,葫芦夜交汤组给予葫芦夜交汤按生药150 mg·kg-1灌胃。结果:与对照组比较,给药后第14天、第21天和第28天模型组、氟西汀组和葫芦夜交汤组大鼠糖水消耗比例减少,差异具有统计学意义(P<0.05);与模型组比较,氟西汀组和葫芦夜交汤组大鼠糖水消耗比例在第21天和第28天时显著增加,差异具有统计学意义(P<0.01),第28天时葫芦夜交汤组大鼠糖水消耗比例高于氟西汀组,差异有统计学意义(P<0.05)。与对照组比较,模型组大鼠水平运动评分在第21天和第28天时显著下降,垂直运动评分在第28天时明显下降,差异均有统计学意义(P<0.01)。与模型组比较,氟西汀组和葫芦夜交汤组在第21天和第28天时水平运动评分显著增加,在第28天时垂直运动评分显著增加,且葫芦夜交汤组水平运动及垂直运动评分均高于氟西汀组,差异均有统计学意义(P<0.05)。与对照组比较,模型组大鼠在水中不动时间显著增加,游动时间及攀爬状态时间显著缩短,差异均有统计学意义(P<0.05);与模型组比较,氟西汀组和葫芦夜交汤组大鼠水中不动时间显著缩短,游动时间显著增加,差异均有统计学意义(P<0.05)。与对照组比较,模型组大鼠脑皮质5-羟色胺(5-hydroxy tryptamin,5-HT)、去甲肾上腺素(norepinephrine,NE)含量明显降低,差异具有统计学意义(P<0.05);与模型组比较,氟西汀组、葫芦夜交汤组大鼠脑皮质5-HT、NE含量明显升高,�
Objective:To observe the influence of Fructus Lagenariae and Caulis Polygoni Multiflori Decoction on behaviors and neurotransmitters in rats with post-stroke depression(PSD).Methods:A total of 80 healthy male SD rats were randomly divided into the control group,the model group,the fluoxetine group and the group of Fructus Lagenariae and Caulis Polygoni Multiflori Decoction,with 20 rats in each group.Except the control group,the other groups used the middle cerebral artery suture method to prepare the stroke rat model,and then used a variety of chronic stimulation to prepare PSD model,while the control group rats did not have any intervention.The rats in control group and model group were given 2.5 mL normal saline by gavage.In the fluoxetine group,the ones were given 2 mg·kg-1 fluoxetine dissolved into 2.5 mL normal saline by gavage.In the group of Fructus Lagenariae and Caulis Polygoni Multiflori Decoction,the ones were given the decoction by gavage with 150 mg·kg-1 crude drug dissolved in 2.5 ml normal saline.Results:Compared with the control group,on the 14 th,21 st and 28 th day after administration respectively,the consumption ratio of sugarcane water in the model group,the fluoxetine group and the group of Fructus Lagenariae and Caulis Polygoni Multiflori Decoction decreased,and the difference was statistically significant(P<0.05).Compared with the model group,the sucrose consumption ratio of rats in the fluoxetine group and the group of Fructus Lagenariae and Caulis Polygoni Multiflori Decoction increased significantly on the 21 st and 28 th day respectively,and the difference was statistically significant(P<0.01).On the 28 th day,the consumption ratio of sugarcane water in the group of Fructus Lagenariae and Caulis Polygoni Multiflori Decoction was higher than that in the fluoxetine group,and the difference was statistically significant(P<0.05).Compared with the control group,the horizontal motor score of the model group decreased significantly on the 21 st and 28 th day respectively,and the vertical mo
作者
郭冬丽
周哲屹
王洁琼
安红伟
卢昌均
GUO Dong-li;ZHOU Zhe-yi;WANG Jie-qiong;AN Hong-wei;LU Chang-jun(Liuzhou Traditional Chinese Medicine Hospital,Liuzhou,Guangxi,China,545001;Guangzhou University of Chinese Medicine,Guangzhou,Guangdong,China,510006)
出处
《河南中医》
2020年第12期1817-1822,共6页
Henan Traditional Chinese Medicine
基金
国家自然科学基金项目(81760902)
广西壮族自治区中医药民族医药继承创新工程立项课题项目(GZZJ13-20)
柳州市科学研究与技术开发计划项目(2014J030407)。
关键词
卒中后抑郁
氟西汀
葫芦夜交汤
大鼠
post-stroke depression(PSD)
fluoxetine
Fructus Lagenariae and Caulis Polygoni Multiflori Decoction
rats
