摘要
目的:探讨血浆miR-136-5p表达水平对口腔鳞状细胞癌(OSCC)患者预后预测的价值。方法:选择2015年1月—2018年10月儋州市人民医院收治的OSCC患者86例及正常对照组50例,采用实时荧光定量PCR技术检测2组患者血浆miR-136-5p表达水平。通过ROC曲线确定血浆miR-136-5p表达水平的最佳截断值,并以此为标准,将OSCC患者分为高miR-136-5p组(n=36)和低miR-136-5p组(n=50),对2组临床病理特征进行比较。应用Kaplan-Meier法绘制生存曲线,采用SPSS 20.0软件包中的单因素及多因素Cox回归模型,分析影响OSCC患者预后不良的危险因素。结果:OSCC组患者血浆miR-136-5p表达水平(1.27±0.51)显著低于对照组(4.38±1.26,P<0.01);血浆miR-136-5p低表达与OSCC患者的临床分期、分化程度、肿瘤直径及颈淋巴结转移有关(P<0.05)。生存分析显示,低miR-136-5p组患者总生存率(48.0%)显著低于高miR-136-5p组(68.4%,P<0.01),低miR-136-5p组患者无进展生存率(28.6%)显著低于高miR-136-5p组患者(57.8%,P<0.01)。多因素Cox回归模型分析显示,临床分期[HR(95%CI)=2.462(1.805~4.193)]、颈淋巴结转移[HR(95%CI)=2.913(2.142~5.827)]及血浆miR-136-5p表达水平<2.82[HR(95%CI)=3.856(3.114~8.205)]是影响OSCC患者预后不良的独立危险因素(P<0.05)。结论:血浆miR-136-5p低表达与OSCC患者生存期短有关,有望作为预测OSCC预后不良的生物标志物。
PURPOSE:To investigate the prognostic value of plasma level of miR-136-5p in patients with oral squamous cell carcinoma(OSCC).METHODS:From January 2015 to October 2018,86 OSCC patients and 50 normal controls were selected from Danzhou People’s Hospital.The expression of miR-136-5p in plasma of the two groups were detected by real-time fluorescence quantitative PCR.The optimal cut-off value of plasma miR-136-5p expression level was determined by ROC curve,and OSCC patients were divided into high miR-136-5p group(n=36)and low miR-136-5p group(n=50).The clinicopathological features of the two groups were compared.Survival curves were drawn by KaplanMeier method.Single factor and multi factor Cox regression models in SPSS 20.0 software package were used to analyze the risk factors of poor prognosis in OSCC patients.RESULTS:The expression level of plasma miR-136-5p in OSCC group(1.27±0.51)was significantly lower than that in the control group(4.38±1.26,P<0.01).Low expression of plasma miR-136-5p was related to the clinical stage,differentiation degree,tumor diameter and cervical neck lymph node metastasis of OSCC(P<0.05).Survival analysis showed that the overall survival rate of low miR-136-5p group(48.0%)was significantly lower than that of high miR-136-5p group(68.4%,P<0.01),and the progression-free survival rate of low miR-136-5p group(28.6%)was significantly lower than that of high miR-136-5p group(57.8%,P<0.01).Multivariate Cox regression model analysis showed that clinical stage[HR(95%CI)=2.462(1.805-4.193)],cervical neck lymph node metastasis[HR(95%CI)=2.913(2.142-5.827)]and plasma miR-136-5p expression<2.82[HR(95%CI)=3.856(3.114-8.205)]were independent risk factors for poor prognosis of patients with OSCC(P<0.05).CONCLUSIONS:Low expression of plasma miR-136-5p is related to short survival time of OSCC patients,which is expected to be a biomarker for predicting poor prognosis of OSCC.
作者
陈秀敏
刘族志
林建能
CHEN Xiu-min;LIU Zu-zhi;LIN Jian-neng(Department of Stomatology,Danzhou people’s Hospital,Danzhou 571799,Hainan Province,China)
出处
《中国口腔颌面外科杂志》
CAS
2020年第6期526-530,共5页
China Journal of Oral and Maxillofacial Surgery
