摘要
目的:研究长链非编码RNA FOXD2-AS1对舌鳞癌(tongue squamous cell carcinoma,TSCC)细胞迁移和侵袭的影响。方法:利用RT-qPCR检测长链非编码RNA(long noncoding RNA,lncRNA)FOXD2-AS1在35对舌鳞癌临床组织样本中的表达,结合第8版UICC口腔癌TNM分期标准,对样本的临床病理特征信息进行分析。根据癌组织与癌旁组织的RT-qPCR检测数据,建立受试者工作特征曲线(receiver operating characteristic curve,ROC)。在舌鳞癌细胞系SCC-9和CAL-27中,应用siRNA敲低FOXD2-AS1,进行Transwell迁移和侵袭实验、克隆形成实验,分别检测其对舌鳞癌细胞迁移、侵袭及增殖的影响,Western免疫印迹实验检测其对下游信号通路分子的影响。采用SPSS 23.0软件包对数据进行统计分析。结果:RT-qPCR结果表明,与癌旁组织相比,lncRNA FOXD2-AS1在舌鳞癌组织中显著高表达,且其高表达与颈淋巴结转移以及不良临床分期相关。ROC曲线下面积(area under curve,AUC)为0.8122,95%CI为0.7141~0.9103,灵敏度和特异度分别为0.6和0.8857,cutoff值为4.122。在SCC-9和CAL-27中敲低FOXD2-AS1后,Transwell迁移和侵袭实验显示,舌鳞癌细胞的迁移和侵袭能力被抑制;克隆形成实验显示,其对舌鳞癌细胞增殖能力无显著影响。Western免疫印迹实验显示,敲低lncRNA FOXD2-AS1后,Wnt/β-catenin信号通路中β-catenin分子表达下调。结论:lncRNA FOXD2-AS1在舌鳞癌中高表达,其高表达与舌鳞癌颈淋巴结转移以及不良临床分期相关,lncRNA FOXD2-AS1可促进舌鳞癌细胞的迁移侵袭能力,并可能通过Wnt/β-catenin信号通路进行调控。
PURPOSE:To investigate the effects of long noncoding RNA FOXD2-AS1 on migration and invasion of tongue squamous cell carcinoma(TSCC).METHODS:RT-qPCR was carried out to validate the expression of lncRNA FOXD2-AS1 in 35 paired clinical TSCC tissues,the relationship between FOXD2-AS1 expression and clinicopathological characteristics was analyzed with the 8 th version of UICC TNM staging criteria.Receiver operating characteristic curve(ROC)was made according to RT-qPCR data.Transwell migration and invasion,colony formation assays were performed to detect migration,invasion and proliferative ability of TSCC cells after FOXD2-AS1 being downregulated in SCC-9 and CAL-27 cell lines.Western blot was conducted to detect the influence that FOXD2-AS1 downregulation might have on downstream molecules.SPSS 23.0 software package was used to analyze the data.RESULTS:Compared to para-carcinoma tongue tissues,lncRNA FOXD2-AS1 was highly expressed in TSCC tissues and its high expression was associated with cervical lymph node metastasis and poor TNM stages.In ROC,area under curve(AUC)was 0.8122,95%CI was 0.7141-0.9103,sensitivity and specificity were 0.6 and 0.8857,respectively,and the value of cutoff was 4.122.After FOXD2-AS1 being downregulated in SCC-9 and CAL-27 cell lines,Transwell migration and invasion assays revealed that migration and invasion of TSCC cells were inhibited,but the proliferative ability remained unchanged.Western blot indicated that the expression ofβ-catenin in Wnt/β-catenin signal pathway downregulated after lncRNA FOXD2-AS1 knockdown.CONCLUSIONS:lncRNA FOXD2-AS1 is highly expressed in TSCC,which promotes migration and invasion of TSCC and is associated with cervical lymph node metastasis and poor TNM stages,probably through modulating Wnt/β-catenin signal pathway.
作者
周光明
梁衍灿
黄子贤
张彬
ZHOU Guang-ming;LIANG Yan-can;HUANG Zi-xian;ZHANG Bin(Department of Oral and Maxillofacial Surgery,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,Guangdong Province,China)
出处
《中国口腔颌面外科杂志》
CAS
2020年第6期495-500,共6页
China Journal of Oral and Maxillofacial Surgery
基金
国家自然科学基金青年项目(81802700)。
