摘要
目的探讨Th1、Th2、CD4^+在肺结核合并慢性乙型肝炎(HBV)患者血清中的表达水平。方法收集陕西省结核病防治院2016年1月至2018年12月收治的肺结核合并HBV患者90例(肺结核合并HBV组);另收集同期收治的单纯肺结核病患者29例(肺结核组)、单纯HBV患者36例(HBV组)、健康体检者50名(健康对照组)。比较各组外周血白细胞介素-2(IL-2)、IL-4、IL-10、肿瘤坏死因子-α(TNF-α)、干扰素-γ(INF-γ)表达水平,比较各组Th1、Th2、CD4^+表达水平。结果肺结核合并HBV组、肺结核组、HBV组IL-2、INF-γ水平明显低于健康对照组,TNF-α、IL-4、IL-10明显高于健康对照组,差异有统计学意义(P<0.05);肺结核合并HBV组IL-2、INF-γ明显低于肺结核组、HBV组,IL-4、IL-10明显高于肺结核组、HBV组,差异有统计学意义(P<0.05);肺结核合并HBV组、肺结核组、HBV组TNF-α水平比较差异无统计学意义(P>0.05)。肺结核合并HBV组、肺结核组、HBV组Th1、CD4^+表达水平明显低于健康对照组,Th2表达水平明显高于健康对照组,差异有统计学意义(P<0.05);肺结核合并HBV组CD4^+、Th1表达水平明显低于肺结核组、HBV组,Th2表达明显高于肺结核组、HBV组,差异有统计学意义(P<0.05)。结论肺结核合并HBV可导致Th1/Th2免疫平衡进一步恶化,IL-2、INF-γ、TNF-α、IL-4、IL-10等细胞因子表达紊乱,CD4^+T淋巴细胞免疫异常,导致病情重症进展。
Objective To investigate the levels of Thl,Th2 and CD4^+in serum of patients with pulmonary tuberculosis with chronic hepatitis B.Methods From January 2016 to December 2018,90 patients with pulmonary tuberculosis with HBV were collected in Shaaxi Provincial Tuberculosis Prevention and Treatment Hospital as pulmonary tuberculosis with HBV group,29 patients with simple pulmonary tuberculosis as pulmonary tuberculosis group,36 patients with HBV as HBV group and 50 healthy controls as healthy control group.The expression levels of IL-2,IL-4,IL-10,TNF-αand INF-γin peripheral blood of each group were compared.Results IL-2 and INF-γlevels in disease groups(pulmonary tuberculosis with HBV group,pulmonary tuberculosis group,HBV group)were significantly lower than those in healthy control group.TNF-α,IL-4 and IL-10 levels were significantly higher than those in healthy control group(P<0.05).IL-2 and INF-γlevels in the pulmonary tuberculosis with HBV group were significantly lower than those in the pulmonary tuberculosis group and HBV group.IL-4 and IL-10 levels in the pulmonary tuberculosis with HBV group were significantly higher than those in the pulmonary tuberculosis group and HBV group(P<0.05).There was no significant difference in the levels of TNF-αin the disease groups(P>0.05).The Thl and CD4^+expression levels of pulmonary tuberculosis with HBV group,pulmonary tuberculosis group,and HBV group were significantly lower than those of the healthy control group,and the Th2 expression level was significantly higher than that of the healthy control group,the difference was statistically significant(P<0.05);The CD4^+and Thl expression level in pulmonary tuberculosis with HBV group was significantly lower than that of pulmonary tuberculosis group and HBV group,and Th2 expression was significantly higher than that of pulmonary tuberculosis group and HBV group,the difference was statistically significant(P<0.05).Conclusion Pulmonary tuberculosis with HBV could lead to the further deterioration of Thl/Th2 immune balance
作者
高瑜
王欣
郭珍
刘红艳
胡萍
王立
GAO Yu;WANG Xin;GUO Zhen;LIU Hong-yan;HU Ping;WANG Li(Shaanxi Provincial Tuberculosis Prevention and Treatment Hospital,Xi'an,Shaanxi 710100,China)
出处
《热带医学杂志》
CAS
2020年第10期1304-1307,共4页
Journal of Tropical Medicine
基金
陕西省科学技术研究发展计划项目(2015SF189)。
