摘要
目的研究宫颈癌(CC)癌组织中畸胎瘤衍生生长因子1(Cripto-1)与哺乳动物雷帕霉素靶蛋白(mTOR)的表达及其临床意义。方法应用荧光实时定量PCR(qRT-PCR)检测2012年1月至2017年5月成都医学院第二附属医院收治的152例CC组织及40例正常宫颈组织中Cripto-1与mTOR表达,统计学分析组间表达差异及两者与临床病理特征及预后的关系。结果CC组织中Cripto-1与mTOR的相对表达量明显高于正常宫颈组织(P<0.05)。CC组织中Cripto-1与mTOR表达呈正相关(r=0.634,P<0.05)。CC组织中Cripto-1、mTOR蛋白与国际妇产科联盟(FIGO)分期、病理分级有关(P均<0.05),与患者年龄、肿瘤的病理类型、浸润深度、是否伴有淋巴结转移无关(P均>0.05)。高Cripto-1表达患者的3年总体生存率(OS)与低Cripto-1表达患者的3年OS差异无统计学意义(P>0.05),而高mTOR表达组3年OS明显低于低mTOR表达组患者(χ^2=5.808,P=0.016)。结论CC组织中Cripto-1与mTOR均表达增加,两者均与FIGO分期、病理分级有关,有可能成为CC诊断、治疗及评估的新分子标志物。
Objective To study the expression and clinical significance of teratocarcinoma-derived growth factor 1(Cripto-1)and mammalian target of rapamycin(mTOR)in cervical cancer(CC)cancer tissues.Methods From January 2012 to May 2017,152 patients with CC in the 2nd Affiliated Hospital of Chengdu Medical College were selected as CC group,and 40 patients with uterine fibroids as control group.The quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the expression of Cripto-1 and mTOR in 152 cases of CC tissues and 40 cases of normal cervical tissues.The expression difference between the two and their relationship with pathological features and prognosis were statistically analyzed.Results The relative expression of Cripto-1 and mTOR in CC tissues was significantly higher than that in normal cervical tissues(P<0.05).There was a significant positive correlation between Cripto-1 and mTOR expression in CC tissues(r=0.634,P<0.05).The expression of Cripto-1 and mTOR protein in CC tissues were associated with International Federation of Gynecology and Obstetrics(FIGO)stage and tumor grade(P<0.05),but not with age,pathological type,depth of invasion and lymph node metastasis(P>0.05).The 3-year overall survival(OS)rate of patients with high Cripto-1 expression was not significantly different from that of patients with low Cripto-1 expression(P>0.05),while the 3-year OS of high mTOR expression group was significantly lower than that of low mTOR expression group(χ^2=5.808,P=0.016).Conclusions The expression of Cripto-1 and mTOR is increased in CC tissues.Both of them are related to FIGO stage and tumor grade,which may become a new molecular marker for diagnosis,treatment and evaluation of CC.
作者
孟侠
杨智玲
向睿
刘海凤
王静依
Meng Xia;Yang Zhiling;Xiang Rui;Liu Haifeng;Wang Jingyi(Department of Obstetrics and Gynecology,the 2nd Affiliated Hospital of Chengdu Medical College,Nuclear Industry 416 Hospital,Chengdu 610051,China)
出处
《中国医师杂志》
CAS
2020年第11期1611-1614,1618,共5页
Journal of Chinese Physician
基金
四川省卫生和计划生育委员会研究课题项目(17PJ250)。
