摘要
目的探讨磷脂酰肌醇-3-激酶(PI3K)、真核细胞转录因子κB(NF-κB)p65蛋白在宫颈癌组织中的表达及其临床意义。方法选取宫颈癌患者62例作为研究对象,提取宫颈癌组织及癌旁组织,采用免疫组织化学染色法检测PI3K和NF-κB p65蛋白的表达情况,对比宫颈癌组织和癌旁组织中REG4蛋白和Sp1蛋白阳性情况,分析PI3K和NF-κB p65蛋白表达与宫颈癌临床病理参数的相关性。结果宫颈癌组织中PI3K、NF-κB p65蛋白阳性表达率为62.90%和69.35%,癌旁正常组织中无阳性表达,两组比较,P<0.05。不同年龄、病理分型、肿瘤直径患者的PI3K、NF-κB p65蛋白阳性表达比较,差异无统计学意义(P>0.05);FIGOⅢ~Ⅳ期患者PI3K和NF-κB p65阳性表达率为76.92%和82.05%,高于FIGOⅠ~Ⅱ期患者的39.13%和47.83%(P<0.05);不同分化程度患者PI3K和NF-κB p65阳性表达率从高到低依次为低分化(83.33%,94.44%)、中分化(61.11%,63.89%)、高分化(25.00%,37.5%)(P<0.05);淋巴结转移患者的PI3K和NF-κB p65阳性表达率分别为86.96%和95.65%,高于无淋巴结转移患者的48.72%和53.85%(P<0.05);相关性分析显示,宫颈癌患者的PI3K和NF-κB p65表达水平与FIGO分期、淋巴结转移呈正相关,与分化程度呈负相关(P<0.05)。结论宫颈癌组织中PI3K、NF-κB p65蛋白阳性表达率均高于癌旁正常组织,并与FIGO分期、淋巴结转移呈正相关,与分化程度呈负相关,对于宫颈癌诊治具有一定的临床意义。
Objective To explore the expressions and clinical significance of phosphatidylinositol-3-kinase(PI3K)and nuclear transcription factorκB(NF-κB)p65 proteins in cervical cancer tissues.Methods A total of 62 patients with cervical cancer were enrolled as the research objects.The cervical cancer and para-carcinoma tissues were collected to detect expressions of PI3K and NF-κB p65 proteins by immunohistochemical staining.The positive REG4 and Sp1 proteins in cervical cancer and para-carcinoma tissues were compared.The correlation between PI3K,NF-κB p65 proteins and clinicopathological parameters was analyzed.Results The differences in positive expression rates of PI3K and NF-κB p65 proteins between cervical cancer tissues and para-carcinoma tissues were statistically significant(62.90%,69.35%vs 0%,0%,P<0.05).There was no significant difference in positive expressions of PI3K and NF-κB p65 proteins among patients with different age,pathological classifications and tumor diameter(P>0.05).The positive expression rates of PI3K and NF-κB p65 in patients with FIGO staging at stagesⅢ~Ⅳwere 76.92%and 82.05%,higher than those at stagesⅠ~Ⅱ(39.13%,47.83%,P<0.05).The positive expression rates of PI3K and NF-κB p65 were the highest in patients with low differentiation(83.33%,94.44%),followed by medium differentiation(61.11%,63.89%)and high differentiation(25.00%,37.5%;P<0.05).The positive expression rates of PI3K and NF-κB p65 in patients with lymph node metastasis were 86.96%and 95.65%,higher than those without metastasis(48.72%,53.85%;P<0.05).Correlation analysis showed that expression levels of PI3K and NF-κB p65 proteins were positively correlated with FIGO staging and lymph node metastasis,while negatively correlated with differentiation degree(P<0.05).Conclusion The positive expression rates of PI3K and NF-κB p65 proteins in cervical cancer tissues are higher than those in normal para-carcinoma tissues,which are positively correlated with FIGO staging and lymph node metastasis,while negatively correlated wi
作者
王秋香
喻春霞
夏小飞
WANG Qiuxiang;YU Chunxia;XIA Xiaofei(Henan Provincial Third People's Hospital,Zhengzhou,450000)
出处
《实用癌症杂志》
2024年第2期190-193,共4页
The Practical Journal of Cancer
