摘要
查尔酮合成酶(chalcone synthase, CHS)是植物类黄酮生物合成途径的第一个关键酶,在植物的次生代谢途径中发挥重要作用。本研究基于课题组构建的甘蔗转录组数据库,挖掘获得7个具有完整编码区的甘蔗ScCHS基因(ScCHS1~ScCHS7)。序列分析结果显示,ScCHS1~Sc CHS7基因大约编码400个氨基酸,7者之间的氨基酸序列相似性介于12.47%~87.22%之间,且其编码蛋白均含有c (Cys)、f (Phe)、h (His)和n (Asn)这4个构成CHS蛋白催化中心的保守残基。系统进化树分析表明,ScCHS1、ScCHS2和ScCHS6聚类在CHS家族的第Ⅵ亚类,ScCHS3和ScCHS4聚在第Ⅲ亚类,ScCHS5聚在第Ⅴ亚类,ScCHS7聚在第Ⅷ亚类。实时荧光定量PCR (quantitative real-time PCR, qRT-PCR)结果显示,接种甘蔗黑穗病菌(Sporisorium scitamineum)后,ScCHS1基因的表达水平在感黑穗病品种‘ROC22’中没有显著变化,但在抗黑穗病品种‘NCo376’中被诱导上调。此外,在低氮胁迫下,ScCHS1基因转录本在‘ROC22’叶片和根部组织中均被诱导上调,表明ScCHS1基因积极响应甘蔗黑穗病菌和低氮胁迫处理。以上结果为深入研究甘蔗ScCHS家族基因的结构和功能提供了参考资料。
Chalcone synthase(CHS) is the first key enzyme in the biosynthesis pathway, which plays an important role in the secondary metabolic pathways of plants. In this study, based on the sugarcane transcriptome database constructed by our research group, seven sugarcane ScCHS genes(ScCHS1~ScCHS7) with complete coding regions were obtained. The results of sequence analysis showed that the Sc CHS1~ScCHS7 gene encoded about 400 amino acids. The similarity of amino acid sequences between these seven genes ranged from 12.47% to 87.22%. Their coding proteins all contained four conserved residues of c(Cys), f(Phe), h(His), and n(Asn), which constituted the catalytic center of CHS protein. Phylogenetic tree analysis indicated that ScCHS1, ScCHS2 and SCCHS6 clustered in subgroup Ⅵ of CHS family, ScCHS3 and ScCHS4 clustered in subgroup Ⅲ, ScCHS5 clustered in subgroup Ⅴ, and ScCHS7 clustered in subgroup Ⅶ. Quantitative real-time PCR(qRT-PCR) results showed that the expression level of ScCHS1 gene did not change significantly in sugarcane smut-susceptible variety ’ROC22’after inoculation with Sporisorium scitamineum, but was induced to increase in resistant variety ’NCo376’. In addition, under low nitrogen stress, the transcripts of Sc CHS1 gene were induced in both leaf and root tissues of’ROC22’. Upregulation of ScCHS1 gene suggested that it responded positively to sugarcane smut fungus and low nitrogen stresses. These results provide basic data for further study on the structure and function of Sc CHS family genes in sugarcane.
作者
王文举
于胜晓
岑光莉
孙婷婷
尤垂淮
娄文月
陈玉凤
杨颖颖
苏亚春
Wang Wenju;Yu Shengxiao;Cen Guangli;Sun Tingting;You Chuihuai;Lou Wenyue;Chen Yufeng;Yang Yingying;Su Yachun(Key Laboratory of Sugarcane Biology and Gienetie Breeding(Fujan),Ministry of Agriculture,Fujian Agriculture and Forstry University,Fuzhou,350002;College of Life Scienees,Fujian Agriculture and Forestry University,Fuzhou,30002;School of Ocean and Biochemical Engineering,Fuqing Branch of Fujian Normal University,Fuqing,350300)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第22期7364-7375,共12页
Molecular Plant Breeding
基金
国家重点研发计划(2018YFD1000503)
福建农林大学科技创新专项基金项目(KFA17173A)
国家自然科学基金项目(31501363)
国家现代农业产业技术体系建设专项(CARS-17)共同资助
关键词
甘蔗
查尔酮合成酶基因
生物信息学分析
表达模式分析
Sugarcane
Chalcone synthase gene
Bioinformatics analysis
Expression pattern analysis
