摘要
甘草(Glycyrrhiza uralensis)是一味大宗中药材,黄酮类活性成分是其主要的药物活性成分之一。茉莉酸是甘草响应非生物胁迫调控类黄酮类活性成分的主要内源激素。JAZ是茉莉酸信号转导途径中的一个重要节点,其通过负向调节茉莉酸的信号转导,参与植物发育、非生物胁迫和对植物激素处理的响应的调节。本研究通过测定聚乙二醇(polyethylene glycol,PEG)6000诱导的干旱胁迫对乌拉尔甘草(Glycyrrhiza uralensis Fisch.)中茉莉酸(jasmonic acid,JA)含量和JAZ基因家族表达水平以及JAZ基因家族的生物信息学分析,筛选受干旱胁迫诱导JAZ基因。研究结果显示:JA主要在甘草地下部分中积累,干旱胁迫明显提升地下部分JA含量,地下和地上部分中PEG处理2h时JA表达量最高。基于对上述材料的转录组测序数据分析,有10个GuJAZ基因具有完整的读码框,且这些JAZ基因具有时空表达差异性。干旱胁迫诱导地下部分中的GuJAZ基因上调,GuJAZ1、GuJAZ3、GuJAZ4、GuJAZ5、GuJAZ 6和GuJAZ 10在处理2h组上调最明显,表达量变化趋势与JA含量变化趋势相似。GuJAZ启动子调控元件分析显示GuJAZ1、GuJAZ5、GuJAZ6、GuJAZ 8和GuJAZ 10基因主要受逆境胁迫和光响应元件调控。本研究为优化甘草栽培体系、提升栽培甘草品质、探究GuJAZ基因在甘草中的功能以及对JA合成及调控机制提供研究基础。
Licorice(Glycyrrhiza uralensis)is a bulk Chinese herb with flavonoid active ingredients as one of its major pharmacological active ingredients.JAZ is an important node in the jasmonic acid(JA)signaling pathway,which is involved in the regulation of plant development,abiotic stress and response to phytohormone treatment by negatively regulating the signal transduction of JA.In this study,we screened for drought stress-induced JAZ genes by measuring the JA content and JAZ gene family expression levels in Ural glycyrrhiza(Glycyrrhiza uralensis Fisch.)by PEG 6000-induced drought stress and bioinformatics analysis of the JAZ gene family.The results showed that JA accumulated mainly in the underground parts of Glycyrrhiza uralensis,and drought stress significantly elevated the JA content in the underground parts,and the highest JA expression was observed in the underground and aboveground parts at 2 h of PEG treatment.Based on the analysis of transcriptome sequencing data of the above materials,there were 10 GuJAZ genes with complete reading frames and these JAZ genes had differential spatial and temporal expression patterns.Drought stress induced the up-regulation of GuJAZ genes in the belowground part,and GuJAZ1,GuJAZ3,GuJAZ4,GuJAZ5,GuJAZ 6,and GuJAZ 10 were most significantly up-regulated in the 2 h-treatment group,and the trend of expression change was similar to that of JA contents.analysis of GuJAZ promoter regulatory elements showed that GuJAZ1,GuJAZ5,GuJAZ6,GuJAZ 8 and GuJAZ 10 genes were mainly regulated by stress and light response elements.This study provides a research basis for optimizing licorice cultivation systems,improving the quality of cultivated licorice,and exploring the functions of GuJAZ genes in licorice as well as the mechanism of JA synthesis and regulation.
作者
石娜娜
姚华
高转转
赵斌
杨茂
沈海涛
SHI Na-Na;YAO Hua;GAO Zhuan-Zhuan;ZHAO Bin;YANG Mao;SHEN Hai-Tao(School of Life Sciences,Shihezi University,Key Laboratory of Phytomedicinal Resources,Ministry of Education,Xinjiang,Key Laboratory of Oasis Town and Mountain-Basin System Ecology Corps,Shihezi,Xinjiang 832003,China;Institute of Pharmaceutical Research,Xinjiang Uygur Autonomous Region,Urumqi 830004)
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2023年第12期1776-1788,共13页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家自然基金项目(No.32260083)
兵团财政科技计划项目(No.2020AA005)
兵团重点领域科技攻关项目-基础研究项目(No.2023CB008-17)
高层次人才科研启动经费项目(No.RCZK2021B31)
石河子大学新品种培育专项(No.YZZX202102)资助
关键词
乌拉尔甘草
GuJAZ
茉莉酸
生物信息学分析
glycyrrhiza uralensisfisch.
jasmonateZIM-domain gujaz
ja
bioinformatics analysis
