摘要
目的:探讨人类微小RNA-9(Hsa-miR-9)在儿童急性淋巴细胞白血病(ALL)中的表达及对尾侧型同源盒基因(CDX2)的调节作用。方法:收集本院近5年诊治的130例初治ALL患儿的临床资料,并选取同时期同年龄段于本院行健康体检的健康人群60例作为对照;采用实时荧光定量PCR(qRT-PCT)分别测定2组儿童Hsa-miR-9的表达量及ALL患儿不同治疗阶段CDX2基因的表达量,分析Hsa-miR-9的表达与患者临床病理特征及患者生存时间的关系,并探讨Hsa-miR-9与CDX2基因表达之间的关系。结果:Hsa-miR-9在ALL患儿中的表达量较健康对照组显著降低(P<0.001);ALL患儿Hsa-miR-9的表达与血清WBC、浸润淋巴结、脾肿大及危险程度分级有关(P<0.05);高表达Hsa-miR-9的ALL患儿的中位生存时间显著长于低表达组(P<0.001);不同治疗阶段ALL患儿的Hsa-miR-9表达均与CDX2基因的表达呈显著相关(rB-ALL初治期=0.54,rB-ALL缓解期=0.94,rT-ALL初治期=0.74)(P<0.05)。结论:Hsa-miR-9在ALL患儿中表达降低,高表达Hsa-miR-9的ALL患儿可获得更长的总体生存时间,Hsa-miR-9在ALL患儿中的表达与CDX2基因密切相关。
Objective:To investigate the expression of Hsa-miR-9 in children with acute lymphoblastic leukemia(ALL)and its relationship with CDX2 gene.Methods:The clinical data of 130 patients with ALL were collected from nearly five years in our hospital,and in the same period 60 healthy children in the same age were selected as control group.Real-time fluorescent quantitative PCR(qRT-PCR)was applied to examine the expression of Hsa-miR-9 and CDX2 gene of the two groups,the relationship between Hsa-miR-9 expression and ALL patients’clinical characteristics,survival time,CDX2 expression were analyzed.Results:The expression level of Hsa-miR-9 in ALL children was significantly lower than that in the healthy control group(P<0.001).The expression of Hsa-miR-9 in ALL children related with serum WBC,infiltrating lymph nodes,splenomegaly,and risk grade(P<0.05).The median survival time of ALL children with high expression of Hsa-miR-9 was significantly longer than that of the ALL children with low expression(P<0.001).Hsa-miR-9 expression significantly correlated with CDX2 gene expression in ALL children at different treatment stages(P<0.05).Conclusion:The expression of Hsa-miR-9 decreases in children with ALL,and ALL children with high expression of Hsa-miR-9 have a longer overall survival time.The expression of hsa-miR-9 in ALL children closely related with CDX2 gene.
作者
王瑞仓
杨洁
张晓霞
高晓晗
WANG Rui-Cang;YANG Jie;ZHANG Xiao-Xia;GAO Xiao-Han(Department of Hematology,Hebei General Hospital,Shijiazhuang 050051,Hebei Province,China)
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2020年第5期1523-1527,共5页
Journal of Experimental Hematology
基金
河北省医学科学研究重点课题计划(ZD20140444)。
