摘要
开发霍山石斛简单重复序列(SSR)分子标记,为霍山石斛遗传多样性和种质资源鉴定的研究提供技术支撑。利用Illumina HiSeq X10平台对霍山石斛叶片进行转录组测序,Microsatellite(MISA)软件分析霍山石斛转录组SSR的分布频率和重复基元的类型特征,软件Primer 5设计引物。从202080条Unigene中搜到43291个SSR位点,分布在36382条Unigene序列中,发生频率为18.00%,平均每6.08 kb含1个SSR位点,共有463种重复基元,单核苷酸重复占主要地位,发生频率为47.62%,在所有重复基元中,A/T出现频率最高(47.06%)。试验共获得13960条SSR引物。
The paper aims to develop simple sequence repeat(SSR)molecular markers for Dendrobium huoshanense to provide technical support for genetic diversity research and germplasm characterization of D.huoshanense.The Illumina HiSeq X10 platform was used to sequence the transcriptome of D.huoshanense leaves.Microsatellite(MISA)software was used to analyze the distributing frequency of transcriptome SSR and the basic characteristics of repeat motifs.Primers were designed using Primer 5.SSR loci(43291)were obtained from 202080 Unigene,distributed in 36382 Unigene(18.00%).There were 463 kinds of repeat motifs existing in D.huoshanense transcriptome.On average,SSR occurred every 6.08 kb in length.Mononucleotid repeats predominated with an occurrence frequency of 47.62%,and A/T was the most frequent one among all the repeat types(47.06%).A total of 13960 primer pairs were designed for marker development.
作者
洪克前
夏维丽
李佩玲
徐函兵
谷会
陈丽
Hong Keqian;Xia Weili;Li Peiling;Xu Hanbing;Gu Hui;Chen Li(South Subtropical Crops Research Institutes,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory for Postharvest Physiology and Technology of Tropical Horticultural Products of Hainan Province,Zhanjiang Guangdong 524091;Xinyang Agriculture and Forestry University,Xinyang Henan 46400)
出处
《中国农学通报》
2020年第27期106-110,共5页
Chinese Agricultural Science Bulletin
基金
河南省科技攻关项目“铁皮石斛容器保湿栽培技术研究”(172102110248)
信阳市科技计划项目“铁皮石斛仿开放组培立体栽培研究”(160033)
中央级公益性科研院所基本科研业务费专项一中国热带农业科学院院级创新团队项目(1630062017032)。
关键词
霍山石斛
转录组
多态性
SSR
标记开发
Dendrobium huoshanense
transcriptome
polymorphism
simple sequence repeat
marker development
