摘要
以铁皮石斛与霍山石斛的正、反交F1代共63株为试验材料,利用SSR标记鉴定杂种真实性。结果表明,在21对石斛基因组引物中,筛选出双亲多态性引物3对,多态性引物占比为14.3%。用此3对引物可鉴定出铁皮石斛×霍山石斛杂交苗中25株真杂种,比例为78.1%,可鉴定出霍山石斛×铁皮石斛杂交苗中28株真杂种,比例为90.3%。
The authenticity of hybrids created by reciprocal crossing between Dendrobium officinale and D.huoshanense was not always apparent.To resolve the confusion,this study applied SSR markers to differentiate them among 63 F1 generation of the hybrids.Three out of 21 pairs of the SSR primers were found to have polymorphic bands,i.e.,14.3%of all,in the parental lines.By using these 3 pairs of primers,25 out of 32 known(D.officinale×D.huoshanense)hybrids were correctly identified rendering apositive identification rate of 78.1%;while,28 out of 31 known(D.huoshanense×D.officinale)hybrids were positively identified with a rate of 90.3%.
出处
《福建农业学报》
CAS
北大核心
2017年第8期870-873,共4页
Fujian Journal of Agricultural Sciences
基金
福建自然科学基金项目(2014J01382
2017J0115)
福建省种业创新与产业化工程项目(FJZZZX-1540)
关键词
铁皮石斛
霍山石斛
正反交
SSR
杂种鉴定
Dendrobium officinale
Dendrobium huoshanense
reciprocal cross
SSR
hybrid identification
