摘要
目的探讨结核T细胞γ-干扰素释放试验(TB-IGRA)联合结核抗体检测在结核病诊断中的应用价值。方法选择本院2015年1月-2019年3月2350例疑似结核病患者进行TB-IGRA及结核抗体的联合检测,并选择其中确诊的结核病患者90例(结核组),非结核呼吸系统患者98例(非结核组)及同期健康体检者60例作为对照组。观察不同性别、年龄组间TB阳性率及T细胞释放IFN-γ含量的差异,探讨TB-IGRA、结核抗体检测结核病的阳性率及联合检测灵敏度和特异度。结果男性结核抗体阳性率高于女性,差异有统计学意义(χ^2=14.42,P<0.001),不同年龄组间结核抗体阳性率差异无统计学意义(χ^2=3.15,P>0.05);男性T细胞释放IFN-γ含量高于女性(UMann-Whitney=597701.00,P<0.001),不同年龄组间T细胞释放IFN-γ含量差异无统计学意义(χ^2 Kruskal-Wallis=2.38,P>0.05)。TB-IGRA试验诊断结核病的灵敏度为82.2%、特异度为90.8%,高于结核抗体的检测;联合结核抗体检测灵敏度和特异度高于单项IGRA、结核抗体检测。结论TB-IGRA联合结核抗体检测在结核病诊断中具有重要价值,诊断阳性率较高,且联合诊断灵敏度和特异度较高。
Objective To investigate the application value of tuberculosis T cellγ-interferon release test(TB-IGRA)combined with tuberculosis antibody detection in the diagnosis of tuberculosis.Methods A total of 2350 suspected tuberculosis patients were selected in our hospital from January 2015 to March 1919 for joint detection of TB-IGRA and tuberculosis antibodies,and 90 cases of tuberculosis patients were selected as tuberculosis group,98 non-tuberculosis patients were selected as the non-tuberculosis group and 60 healthy subjects in the same period were selected as the control group.The differences of TB positive rate and IFN-γrelease from T cells were observed.The positive rate of TB-IGRA and tuberculosis antibody detection and the sensitivity and specificity of combined detection were investigated.Results The positive rate of tuberculosis antibody in males was higher than that in females,and the difference was statistically significant(χ^2=14.42,P<0.001).There was no statistical significance on the difference in the positive rate of tuberculosis antibody among different age groups(χ^2=3.15,P>0.05);the release of IFN-γin T cells was higher in male than that in female(UMann-Whitney=597701.00,P<0.001).There was no statistical significance on the difference in the release of IFN-γbetween T cells in different age groups(χ^2 Kruskal-Wallis=2.38,P>0.05).The sensitivity of TB-IGRA test for diagnosis of tuberculosis was 82.2%,and the specificity was 90.8%,which was higher than that of tuberculosis antibody.The sensitivity and specificity of combined tuberculosis antibody detection were higher than single IGRA and tuberculosis antibody detection.Conclusion The detection of TB-IGRA combined with tuberculosis antibody is of great value in the diagnosis of tuberculosis.The positive rate of diagnosis is high,and the sensitivity and specificity of combined diagnosis are high.
作者
王华
党丽君
甄拴平
WANG Hua;DANG Li-jun;ZHEN Shuan-ping(Department of Clinical Laboratory,Baoji Hospital of Traditional Chinese Medicine,Baoji,Shaanxi 721001,China)
出处
《中国卫生检验杂志》
CAS
2020年第19期2379-2381,共3页
Chinese Journal of Health Laboratory Technology
关键词
结核T细胞γ-干扰素释放试验
结核抗体
结核病
诊断价值
Tuberculosis T cellγ-interferon release test
Tuberculosis antibody
Tuberculosis
Diagnostic value
